1s8f
From Proteopedia
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| - | [[Image:1s8f.gif|left|200px]] | + | [[Image:1s8f.gif|left|200px]] |
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| - | '''Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II''' | + | {{Structure |
| + | |PDB= 1s8f |SIZE=350|CAPTION= <scene name='initialview01'>1s8f</scene>, resolution 1.77Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> and <scene name='pdbligand=BEZ:BENZOIC ACID'>BEZ</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= RAB9A, RAB9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 Canis lupus familiaris]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S8F is a [ | + | 1S8F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8F OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II., Wittmann JG, Rudolph MG, FEBS Lett. 2004 Jun 18;568(1-3):23-9. PMID:[http:// | + | Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II., Wittmann JG, Rudolph MG, FEBS Lett. 2004 Jun 18;568(1-3):23-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15196914 15196914] |
[[Category: Canis lupus familiaris]] | [[Category: Canis lupus familiaris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: vesicular trafficking]] | [[Category: vesicular trafficking]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:50 2008'' |
Revision as of 12:01, 20 March 2008
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| , resolution 1.77Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , and | ||||||
| Gene: | RAB9A, RAB9 (Canis lupus familiaris) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II
Overview
The small GTPase Rab9 is an essential regulator of vesicular transport from the late endosome to the trans-Golgi network, as monitored by the redirection of the mannose-6-phosphate receptors. The crystal structure of Rab9 complexed to GDP, Mg(2+), and Sr(2+) reveals a unique dimer formed by an intermolecular beta-sheet that buries the switch I regions. Surface area and shape complementarity calculations suggest that Rab9 dimers can form an inactive, membrane-bound pool of Rab9 . GDP that is independent of GDI. Mg(2+)-bound Rab9 represents an inactive state, but Sr(2+)-bound Rab9 . GDP displays activated switch region conformations, mimicking those of the GTP state. A hydrophobic tetrad is formed resembling an effector-discriminating epitope found only in GTP-bound Rab proteins.
About this Structure
1S8F is a Single protein structure of sequence from Canis lupus familiaris. Full crystallographic information is available from OCA.
Reference
Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II., Wittmann JG, Rudolph MG, FEBS Lett. 2004 Jun 18;568(1-3):23-9. PMID:15196914
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