4j6y
From Proteopedia
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| - | + | ==Hfq from Pseudomonas aeruginosa crystallized in GTP presence== | |
| - | === | + | <StructureSection load='4j6y' size='340' side='right' caption='[[4j6y]], [[Resolution|resolution]] 2.14Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4j6y]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J6Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J6Y FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u1s|1u1s]], [[1u1t|1u1t]], [[4j5y|4j5y]], [[4j6w|4j6w]], [[4j6x|4j6x]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hfq, PA4944 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 Pseudomonas aeruginosa PAO1])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j6y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j6y RCSB], [http://www.ebi.ac.uk/pdbsum/4j6y PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Hfq protein forms a doughnut-shaped homohexamer that possesses RNA-binding activity. There are two distinct RNA-binding surfaces located on the proximal and the distal sides of the hexamer. The proximal side is involved in the binding of mRNA and small noncoding RNAs (sRNAs), while the distal side has an affinity for A-rich RNA sequences. In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography. ATP and ADPNP have been located in the distal R-site, which is a site for poly(A) RNA binding. UTP has been found in the so-called lateral RNA-binding site at the proximal surface. CTP has been found in both the distal R-site and the proximal U-binding site. GTP did not form a complex with Hfq under the conditions tested. The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface. | ||
| - | + | Hfq binds ribonucleotides in three different RNA-binding sites.,Murina V, Lekontseva N, Nikulin A Acta Crystallogr D Biol Crystallogr. 2013 Aug;69(Pt 8):1504-13. doi:, 10.1107/S090744491301010X. Epub 2013 Jul 18. PMID:23897473<ref>PMID:23897473</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Pseudomonas aeruginosa pao1]] | [[Category: Pseudomonas aeruginosa pao1]] | ||
| - | [[Category: Lekontseva, N | + | [[Category: Lekontseva, N]] |
| - | [[Category: Murina, V | + | [[Category: Murina, V]] |
| - | [[Category: Nikulin, A D | + | [[Category: Nikulin, A D]] |
[[Category: Lsm]] | [[Category: Lsm]] | ||
[[Category: Mrna]] | [[Category: Mrna]] | ||
Revision as of 13:03, 21 December 2014
Hfq from Pseudomonas aeruginosa crystallized in GTP presence
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