1s8k
From Proteopedia
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| - | [[Image:1s8k.gif|left|200px]] | + | [[Image:1s8k.gif|left|200px]] |
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| - | '''Solution Structure of BmKK4, A Novel Potassium Channel Blocker from Scorpion Buthus martensii Karsch, 25 structures''' | + | {{Structure |
| + | |PDB= 1s8k |SIZE=350|CAPTION= <scene name='initialview01'>1s8k</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NH2:AMINO GROUP'>NH2</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Solution Structure of BmKK4, A Novel Potassium Channel Blocker from Scorpion Buthus martensii Karsch, 25 structures''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1S8K is a [ | + | 1S8K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8K OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins., Zhang N, Chen X, Li M, Cao C, Wang Y, Wu G, Hu G, Wu H, Biochemistry. 2004 Oct 5;43(39):12469-76. PMID:[http:// | + | Solution structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins., Zhang N, Chen X, Li M, Cao C, Wang Y, Wu G, Hu G, Wu H, Biochemistry. 2004 Oct 5;43(39):12469-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15449936 15449936] |
[[Category: Mesobuthus martensii]] | [[Category: Mesobuthus martensii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: alpha/beta scaffold]] | [[Category: alpha/beta scaffold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:56 2008'' |
Revision as of 12:01, 20 March 2008
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Solution Structure of BmKK4, A Novel Potassium Channel Blocker from Scorpion Buthus martensii Karsch, 25 structures
Overview
BmKK4 is a 30 amino acid peptide purified from the venom of the Chinese scorpion Buthus martensi Karsch. It has been classified as the first member of scorpion toxin subfamily alpha-KTx 17. The 3D structure of BmKK4 in solution has been determined by 2D NMR spectroscopy. This toxin adopts a common alpha/beta-motif, but shows a distinctive local conformation. The most novel feature is that the regular arrangements of the side chains of the residues involved in the beta-sheet of BmKK4 are distorted by a classic beta-bulge structure, which involves two residues (Asp18 and Arg19) in the first strand opposite a single residue (Tyr26) in the second strand. The bulge produces two main changes in the structure of the antiparallel beta-sheet: (1) It disrupts the normal alteration of the side chain direction; the side chain of Asp18 turns over to form a salt bridge with that of Arg19. (2) It accentuates the twist of the sheet, and alters the direction of the antiparallel beta-sheet. The unusual structural feature of the toxin is attributed to the shorter peptide segment (Leu15-Arg19) between the third and fourth Cys residues and two unique residues (Asp18 and Arg19) at the position preceding the fourth Cys. In addition, the lower affinity of the peptide for the Kv channel is correlated to the structural features: residue Arg19 instead of a Lys residue at the critical position for binding and the salt bridge formed between residues Arg19 and Asp18.
About this Structure
1S8K is a Single protein structure of sequence from Mesobuthus martensii. Full crystallographic information is available from OCA.
Reference
Solution structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins., Zhang N, Chen X, Li M, Cao C, Wang Y, Wu G, Hu G, Wu H, Biochemistry. 2004 Oct 5;43(39):12469-76. PMID:15449936
Page seeded by OCA on Thu Mar 20 14:01:56 2008
Categories: Mesobuthus martensii | Single protein | Cao, C. | Chen, X. | Hu, G. | Li, M. | Wang, Y. | Wu, H. | Zhang, N. | NH2 | Alpha/beta scaffold
