1sa3
From Proteopedia
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| - | [[Image:1sa3.gif|left|200px]] | + | [[Image:1sa3.gif|left|200px]] |
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| - | '''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site''' | + | {{Structure |
| + | |PDB= 1sa3 |SIZE=350|CAPTION= <scene name='initialview01'>1sa3</scene>, resolution 1.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] | ||
| + | |GENE= MSPIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=479 Moraxella sp.]) | ||
| + | }} | ||
| + | |||
| + | '''An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SA3 is a [ | + | 1SA3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moraxella_sp. Moraxella sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SA3 OCA]. |
==Reference== | ==Reference== | ||
| - | An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site., Xu QS, Kucera RB, Roberts RJ, Guo HC, Structure. 2004 Sep;12(9):1741-7. PMID:[http:// | + | An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site., Xu QS, Kucera RB, Roberts RJ, Guo HC, Structure. 2004 Sep;12(9):1741-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341737 15341737] |
[[Category: Moraxella sp.]] | [[Category: Moraxella sp.]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:02:17 2008'' |
Revision as of 12:02, 20 March 2008
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| , resolution 1.95Å | |||||||
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| Ligands: | |||||||
| Gene: | MSPIR (Moraxella sp.) | ||||||
| Activity: | Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site
Overview
Most well-known restriction endonucleases recognize palindromic DNA sequences and are classified as Type IIP. Due to the recognition and cleavage symmetry, Type IIP enzymes are usually found to act as homodimers in forming 2-fold symmetric enzyme-DNA complexes. Here we report an asymmetric complex of the Type IIP restriction enzyme MspI in complex with its cognate recognition sequence. Unlike any other Type IIP enzyme reported to date, an MspI monomer and not a dimer binds to a palindromic DNA sequence. The enzyme makes specific contacts with all 4 base pairs in the recognition sequence, by six direct and five water-mediated hydrogen bonds and numerous van der Waal contacts. This MspI-DNA structure represents the first example of asymmetric recognition of a palindromic DNA sequence by two different structural motifs in one polypeptide. A few possible pathways are discussed for MspI to cut both strands of DNA, either as a monomer or dimer.
About this Structure
1SA3 is a Single protein structure of sequence from Moraxella sp.. Full crystallographic information is available from OCA.
Reference
An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site., Xu QS, Kucera RB, Roberts RJ, Guo HC, Structure. 2004 Sep;12(9):1741-7. PMID:15341737
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