4k2j
From Proteopedia
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| - | + | ==Decameric ring structure of KSHV (HHV-8) latency-associated nuclear antigen (LANA) DNA binding domain== | |
| - | + | <StructureSection load='4k2j' size='340' side='right' caption='[[4k2j]], [[Resolution|resolution]] 2.05Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4k2j]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_8 Human herpesvirus 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K2J FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF73 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37296 Human herpesvirus 8])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k2j RCSB], [http://www.ebi.ac.uk/pdbsum/4k2j PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | LANA is the KSHV-encoded terminal repeat binding protein essential for viral replication and episome maintenance during latency. We have determined the X-ray crystal structure of LANA C-terminal DNA binding domain (LANADBD) to reveal its capacity to form a decameric ring with an exterior DNA binding surface. The dimeric core is structurally similar to EBV EBNA1 with an N-terminal arm that regulates DNA binding and is required for replication function. The oligomeric interface between LANA dimers is dispensable for single site DNA binding, but is required for cooperative DNA binding, replication function, and episome maintenance. We also identify a basic patch opposite of the DNA binding surface that is responsible for the interaction with BRD proteins and contributes to episome maintenance function. The structural features of LANADBD suggest a novel mechanism of episome maintenance through DNA-binding induced oligomeric assembly. | ||
| - | + | Molecular Basis for Oligomeric-DNA Binding and Episome Maintenance by KSHV LANA.,Domsic JF, Chen HS, Lu F, Marmorstein R, Lieberman PM PLoS Pathog. 2013 Oct;9(10):e1003672. doi: 10.1371/journal.ppat.1003672. Epub, 2013 Oct 17. PMID:24146617<ref>PMID:24146617</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Human herpesvirus 8]] | [[Category: Human herpesvirus 8]] | ||
| - | [[Category: Domsic, J F | + | [[Category: Domsic, J F]] |
| - | [[Category: Marmorstein, R | + | [[Category: Marmorstein, R]] |
[[Category: Dna binding]] | [[Category: Dna binding]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
Revision as of 14:34, 21 December 2014
Decameric ring structure of KSHV (HHV-8) latency-associated nuclear antigen (LANA) DNA binding domain
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