4k92

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{{STRUCTURE_4k92| PDB=4k92 | SCENE= }}
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==A Cryptic TOG Domain with a Distinct Architecture Underlies CLASP-Dependent Bipolar Spindle Formation==
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===A Cryptic TOG Domain with a Distinct Architecture Underlies CLASP-Dependent Bipolar Spindle Formation===
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<StructureSection load='4k92' size='340' side='right' caption='[[4k92]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23727231}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4k92]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K92 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K92 FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CLASP1, KIAA0622, MAST1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k92 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k92 RCSB], [http://www.ebi.ac.uk/pdbsum/4k92 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CLASP is a key regulator of microtubule (MT) dynamics and bipolar mitotic spindle structure with CLASP mutants displaying a distinctive monopolar spindle phenotype. It has been postulated that cryptic TOG domains underlie CLASP's ability to regulate MT dynamics. Here, we report the crystal structure of a cryptic TOG domain (TOG2) from human CLASP1, demonstrating the presence of a TOG array in the CLASP family. Strikingly, CLASP1 TOG2 exhibits a convex architecture across the tubulin-binding surface that contrasts with the flat tubulin-binding surface of XMAP215 family TOG domains. Mutations in key conserved TOG2 determinants abrogate the ability of CLASP mutants to rescue bipolar spindle formation in Drosophila cells depleted of endogenous CLASP. These findings highlight the common mechanistic use of TOG domains in XMAP215 and CLASP families to regulate MT dynamics and suggest that differential TOG domain architecture may confer distinct functions to these critical cytoskeletal regulators.
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==Function==
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A Cryptic TOG Domain with a Distinct Architecture Underlies CLASP-Dependent Bipolar Spindle Formation.,Leano JB, Rogers SL, Slep KC Structure. 2013 May 29. pii: S0969-2126(13)00130-5. doi:, 10.1016/j.str.2013.04.018. PMID:23727231<ref>PMID:23727231</ref>
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[[http://www.uniprot.org/uniprot/CLAP1_HUMAN CLAP1_HUMAN]] Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.<ref>PMID:11290329</ref> <ref>PMID:12837247</ref> <ref>PMID:15631994</ref> <ref>PMID:16866869</ref> <ref>PMID:16914514</ref> <ref>PMID:17543864</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4k92]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K92 OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023727231</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Leano, J B.]]
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[[Category: Leano, J B]]
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[[Category: Rogers, S L.]]
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[[Category: Rogers, S L]]
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[[Category: Slep, K C.]]
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[[Category: Slep, K C]]
[[Category: Cytoplasm microtubule cytoskeleton]]
[[Category: Cytoplasm microtubule cytoskeleton]]
[[Category: Heat-repeat tog domain]]
[[Category: Heat-repeat tog domain]]

Revision as of 14:37, 21 December 2014

A Cryptic TOG Domain with a Distinct Architecture Underlies CLASP-Dependent Bipolar Spindle Formation

4k92, resolution 2.00Å

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