1scj
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1scj.gif|left|200px]] | + | [[Image:1scj.gif|left|200px]] |
| - | + | ||
| - | '''CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX''' | + | {{Structure |
| + | |PDB= 1scj |SIZE=350|CAPTION= <scene name='initialview01'>1scj</scene>, resolution 2.00Å | ||
| + | |SITE= <scene name='pdbsite=AVE:Active+Site+Mutation+Is+SER+221+CYS+That+Blocks+The+Prot+...'>AVE</scene> | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1SCJ is a [ | + | 1SCJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCJ OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution., Jain SC, Shinde U, Li Y, Inouye M, Berman HM, J Mol Biol. 1998 Nov 20;284(1):137-44. PMID:[http:// | + | The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution., Jain SC, Shinde U, Li Y, Inouye M, Berman HM, J Mol Biol. 1998 Nov 20;284(1):137-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9811547 9811547] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 21: | Line 30: | ||
[[Category: subtilisin e - propeptide]] | [[Category: subtilisin e - propeptide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:03:17 2008'' |
Revision as of 12:03, 20 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Subtilisin, with EC number 3.4.21.62 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX
Overview
We report here the crystallographic structure determination of an autoprocessed (Ser221Cys)-subtilisin E-propeptide complex at 2.0 A resolution. The subtilisin domain sequence has a single substitution (Ser221Cys) which has been shown to block the maturation process prior to degradation of the propeptide domain (77 residues) that acts as an intramolecular chaperon. This mutation, however, did not prevent the enzyme from cleaving its propeptide domain with a 60-80% efficiency. The current determination is the first example of a subtilisin E-propeptide complex which has been autoprocessed. A previous structure determination of a BPN'-prosegment complex has been reported in which the subtilisin domain was extensively mutated and a calcium binding loop was deleted. Further, in this earlier determination, the complex was formed by the addition of separately expressed propeptide domain. The structure determination reported here provides additional information about the nature of the interaction between the subtilisin and propeptide domains in this complex.
About this Structure
1SCJ is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution., Jain SC, Shinde U, Li Y, Inouye M, Berman HM, J Mol Biol. 1998 Nov 20;284(1):137-44. PMID:9811547
Page seeded by OCA on Thu Mar 20 14:03:17 2008
