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1se9
From Proteopedia
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| - | [[Image:1se9.gif|left|200px]] | + | [[Image:1se9.gif|left|200px]] |
| - | + | ||
| - | '''Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana''' | + | {{Structure |
| + | |PDB= 1se9 |SIZE=350|CAPTION= <scene name='initialview01'>1se9</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= At3g01050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1SE9 is a [ | + | 1SE9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE9 OCA]. |
==Reference== | ==Reference== | ||
| - | Cell-free protein production and labeling protocol for NMR-based structural proteomics., Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL, Nat Methods. 2004 Nov;1(2):149-53. Epub 2004 Oct 21. PMID:[http:// | + | Cell-free protein production and labeling protocol for NMR-based structural proteomics., Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL, Nat Methods. 2004 Nov;1(2):149-53. Epub 2004 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15782178 15782178] |
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 18: | Line 27: | ||
[[Category: Volkman, B F.]] | [[Category: Volkman, B F.]] | ||
[[Category: cell-free]] | [[Category: cell-free]] | ||
| - | [[Category: center for eukaryotic structural | + | [[Category: center for eukaryotic structural genomic]] |
[[Category: cesg]] | [[Category: cesg]] | ||
[[Category: plant protein]] | [[Category: plant protein]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: ubiquitin-like]] | [[Category: ubiquitin-like]] | ||
[[Category: wheat germ]] | [[Category: wheat germ]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:03:47 2008'' |
Revision as of 12:03, 20 March 2008
| |||||||
| Gene: | At3g01050 (Arabidopsis thaliana) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana
Overview
Structural proteomics requires robust, scalable methods. Here we describe a wheat germ cell-free platform for protein production that supports efficient NMR structural studies of eukaryotic proteins and offers advantages over cell-based methods. To illustrate this platform, we describe its application to a specific target (At3g01050.1) from Arabidopsis thaliana. After cloning the target gene into a specialized plasmid, we carry out a small-scale (50 mul) in vitro sequential transcription and translation trial to ascertain the level of protein production and solubility. Next, we prepare mRNA for use in a 4-ml semicontinuous cell-free translation reaction to incorporate (15)N-labeled amino acids into a protein sample that we purify and test for suitability for NMR structural analysis. We then repeat the cell-free approach with (13)C,(15)N-labeled amino acids to prepare a doubly labeled sample. The three-dimensional (3D) structure of At3g01050.1 shows that this protein is an unusual member of the beta-grasp protein family.
About this Structure
1SE9 is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Cell-free protein production and labeling protocol for NMR-based structural proteomics., Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL, Nat Methods. 2004 Nov;1(2):149-53. Epub 2004 Oct 21. PMID:15782178
Page seeded by OCA on Thu Mar 20 14:03:47 2008
Categories: Arabidopsis thaliana | Single protein | CESG, Center for Eukaryotic Structural Genomics. | Lytle, B L. | Peterson, F C. | Volkman, B F. | Cell-free | Center for eukaryotic structural genomic | Cesg | Plant protein | Protein structure initiative | Psi | Structural genomic | Ubiquitin-like | Wheat germ
