1sfe
From Proteopedia
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| - | [[Image:1sfe.gif|left|200px]] | + | [[Image:1sfe.gif|left|200px]] |
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| - | '''ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI''' | + | {{Structure |
| + | |PDB= 1sfe |SIZE=350|CAPTION= <scene name='initialview01'>1sfe</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Methylated-DNA--[protein]-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.63 2.1.1.63] | ||
| + | |GENE= ADAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SFE is a [ | + | 1SFE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFE OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli., Moore MH, Gulbis JM, Dodson EJ, Demple B, Moody PC, EMBO J. 1994 Apr 1;13(7):1495-501. PMID:[http:// | + | Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli., Moore MH, Gulbis JM, Dodson EJ, Demple B, Moody PC, EMBO J. 1994 Apr 1;13(7):1495-501. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8156986 8156986] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Methylated-DNA--[protein]-cysteine S-methyltransferase]] | [[Category: Methylated-DNA--[protein]-cysteine S-methyltransferase]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:04:11 2008'' |
Revision as of 12:04, 20 March 2008
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| , resolution 2.1Å | |||||||
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| Gene: | ADAC (Escherichia coli) | ||||||
| Activity: | [protein-cysteine_S-methyltransferase Methylated-DNA--[protein]-cysteine S-methyltransferase], with EC number 2.1.1.63 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI
Overview
The mutagenic and carcinogenic effects of simple alkylating agents are mainly due to methylation at the O6 position of guanine in DNA. O6-methylguanine directs the incorporation of either thymine or cytosine without blocking DNA replication, resulting in GC to AT transition mutations. In prokaryotic and eukaryotic cells antimutagenic repair is effected by direct reversal of this DNA damage. A suicidal methyltransferase repair protein removes the methyl group from DNA to one of its own cysteine residues. The resulting self-methylation of the active site cysteine renders the protein inactive. Here we report the X-ray structure of the 19 kDa C-terminal domain of the Escherichia coli ada gene product, the prototype of these suicidal methyltransferases. In the crystal structure the active site cysteine is buried. We propose a model for the significant conformational change that the protein must undergo in order to bind DNA and effect methyl transfer.
About this Structure
1SFE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli., Moore MH, Gulbis JM, Dodson EJ, Demple B, Moody PC, EMBO J. 1994 Apr 1;13(7):1495-501. PMID:8156986[[Category: Methylated-DNA--[protein]-cysteine S-methyltransferase]]
Page seeded by OCA on Thu Mar 20 14:04:11 2008
