4mmr

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{{STRUCTURE_4mmr| PDB=4mmr | SCENE= }}
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==Crystal Structure of Prefusion-stabilized RSV F Variant Cav1 at pH 9.5==
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===Crystal Structure of Prefusion-stabilized RSV F Variant Cav1 at pH 9.5===
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<StructureSection load='4mmr' size='340' side='right' caption='[[4mmr]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24179220}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4mmr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Hrsva Hrsva]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MMR FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jhw|4jhw]], [[4mmq|4mmq]], [[4mms|4mms]], [[4mmt|4mmt]], [[4mmu|4mmu]], [[4mmv|4mmv]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11259 HRSVA]), F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mmr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mmr RCSB], [http://www.ebi.ac.uk/pdbsum/4mmr PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Respiratory syncytial virus (RSV) is the leading cause of hospitalization for children under 5 years of age. We sought to engineer a viral antigen that provides greater protection than currently available vaccines and focused on antigenic site O, a metastable site specific to the prefusion state of the RSV fusion (F) glycoprotein, as this site is targeted by extremely potent RSV-neutralizing antibodies. Structure-based design yielded stabilized versions of RSV F that maintained antigenic site O when exposed to extremes of pH, osmolality, and temperature. Six RSV F crystal structures provided atomic-level data on how introduced cysteine residues and filled hydrophobic cavities improved stability. Immunization with site O-stabilized variants of RSV F in mice and macaques elicited levels of RSV-specific neutralizing activity many times the protective threshold.
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==Function==
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Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus.,McLellan JS, Chen M, Joyce MG, Sastry M, Stewart-Jones GB, Yang Y, Zhang B, Chen L, Srivatsan S, Zheng A, Zhou T, Graepel KW, Kumar A, Moin S, Boyington JC, Chuang GY, Soto C, Baxa U, Bakker AQ, Spits H, Beaumont T, Zheng Z, Xia N, Ko SY, Todd JP, Rao S, Graham BS, Kwong PD Science. 2013 Nov 1;342(6158):592-8. doi: 10.1126/science.1243283. PMID:24179220<ref>PMID:24179220</ref>
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[[http://www.uniprot.org/uniprot/FUS_HRSVA FUS_HRSVA]] Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.<ref>PMID:12663767</ref> <ref>PMID:18216092</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4mmr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MMR OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:024179220</ref><references group="xtra"/><references/>
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__TOC__
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[[Category: Graham, B S.]]
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</StructureSection>
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[[Category: Joyce, M G.]]
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[[Category: Bpt4]]
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[[Category: Kwong, P D.]]
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[[Category: Hrsva]]
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[[Category: McLellan, J S.]]
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[[Category: Graham, B S]]
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[[Category: Sastry, M.]]
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[[Category: Joyce, M G]]
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[[Category: Stewart-Jones, G B.E.]]
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[[Category: Kwong, P D]]
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[[Category: Yang, Y.]]
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[[Category: McLellan, J S]]
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[[Category: Sastry, M]]
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[[Category: Stewart-Jones, G B.E]]
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[[Category: Yang, Y]]
[[Category: Fusion]]
[[Category: Fusion]]
[[Category: Membrane]]
[[Category: Membrane]]
[[Category: Viral protein]]
[[Category: Viral protein]]

Revision as of 15:07, 21 December 2014

Crystal Structure of Prefusion-stabilized RSV F Variant Cav1 at pH 9.5

4mmr, resolution 3.10Å

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