4k8z

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{{STRUCTURE_4k8z| PDB=4k8z | SCENE= }}
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==KOD Polymerase in binary complex with dsDNA==
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===KOD Polymerase in binary complex with dsDNA===
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<StructureSection load='4k8z' size='340' side='right' caption='[[4k8z]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23733496}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4k8z]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_kodakarensis_kod1 Thermococcus kodakarensis kod1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K8Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K8Z FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1wns|1wns]], [[1qht|1qht]], [[4k8x|4k8x]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pol, pol TK0001, TK0001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69014 Thermococcus kodakarensis KOD1])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k8z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k8z RCSB], [http://www.ebi.ac.uk/pdbsum/4k8z PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Replicate it: Structures of KOD and 9 degrees N DNA polymerases, two enzymes that are widely used to replicate DNA with highly modified nucleotides, were solved at high resolution in complex with primer/template duplex. The data elucidate substrate interaction of the two enzymes and pave the way for further optimisation of the enzymes and substrates.
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==Function==
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Structures of KOD and 9 degrees N DNA Polymerases Complexed with Primer Template Duplex.,Bergen K, Betz K, Welte W, Diederichs K, Marx A Chembiochem. 2013 Jun 17;14(9):1058-62. doi: 10.1002/cbic.201300175. Epub 2013, Jun 3. PMID:23733496<ref>PMID:23733496</ref>
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[[http://www.uniprot.org/uniprot/DPOL_PYRKO DPOL_PYRKO]] Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein. PI-PkoI recognizes 5'-GATTTAGATCCCTGTACC-3' and PI-PkoII recognizes 5'-CAGCTACTACGGTTAC-3'.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4k8z]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_kodakarensis_kod1 Thermococcus kodakarensis kod1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K8Z OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:023733496</ref><references group="xtra"/><references/>
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*[[DNA polymerase|DNA polymerase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: DNA-directed DNA polymerase]]
[[Category: DNA-directed DNA polymerase]]
[[Category: Thermococcus kodakarensis kod1]]
[[Category: Thermococcus kodakarensis kod1]]
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[[Category: Bergen, K.]]
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[[Category: Bergen, K]]
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[[Category: Betz, K.]]
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[[Category: Betz, K]]
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[[Category: Diederichs, K.]]
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[[Category: Diederichs, K]]
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[[Category: Marx, A.]]
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[[Category: Marx, A]]
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[[Category: Welte, W.]]
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[[Category: Welte, W]]
[[Category: Dna polymerase]]
[[Category: Dna polymerase]]
[[Category: Transferase-dna complex]]
[[Category: Transferase-dna complex]]

Revision as of 15:07, 21 December 2014

KOD Polymerase in binary complex with dsDNA

4k8z, resolution 2.29Å

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