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4mgn

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{{STRUCTURE_4mgn| PDB=4mgn | SCENE= }}
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==Co-crystal structure of the G. kaustophilus glyQS T box riboswitch Stem I in complex with tRNA==
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===Co-crystal structure of the G. kaustophilus glyQS T box riboswitch Stem I in complex with tRNA===
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<StructureSection load='4mgn' size='340' side='right' caption='[[4mgn]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24095061}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4mgn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MGN FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mgm|4mgm]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mgn RCSB], [http://www.ebi.ac.uk/pdbsum/4mgn PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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T box riboswitches are cis-acting RNA elements that bind to tRNA and sense its aminoacylation state to influence gene expression. Here, we present the 3.2 A resolution X-ray crystal structures of the T box Stem I-tRNA complex and tRNA, in isolation. T box Stem I forms an arched conformation with extensive intermolecular contacts to two key points of tRNA, the anticodon and D/T-loops. Free and complexed tRNA exist in significantly different conformations, with the contacts stabilizing flexible D/T-loops and a rearrangement of the D-loop. Using a designed T box RNA/tRNA system, we demonstrate that the T box riboswitch monitors the length and orientation of two essential contacts. Length or orientation mismatches engineered into the T box riboswitch and tRNA disrupt the complex, whereas simultaneous insertion of full helical turns realigns the interfaces and restores interaction between artificially elongated T box riboswitch and tRNA molecules.
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==About this Structure==
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Structural Determinants for Geometry and Information Decoding of tRNA by T Box Leader RNA.,Grigg JC, Ke A Structure. 2013 Oct 1. pii: S0969-2126(13)00346-8. doi:, 10.1016/j.str.2013.09.001. PMID:24095061<ref>PMID:24095061</ref>
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[[4mgn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MGN OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024095061</ref><references group="xtra"/><references/>
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</div>
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[[Category: Grigg, J C.]]
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[[Category: Ke, A.]]
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==See Also==
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*[[Riboswitch|Riboswitch]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Grigg, J C]]
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[[Category: Ke, A]]
[[Category: Base stacking]]
[[Category: Base stacking]]
[[Category: Rna]]
[[Category: Rna]]

Revision as of 15:11, 21 December 2014

Co-crystal structure of the G. kaustophilus glyQS T box riboswitch Stem I in complex with tRNA

4mgn, resolution 3.20Å

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