1sgv

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[[Image:1sgv.gif|left|200px]]<br /><applet load="1sgv" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1sgv.gif|left|200px]]
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caption="1sgv, resolution 1.90&Aring;" />
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'''STRUCTURE OF TRNA PSI55 PSEUDOURIDINE SYNTHASE (TRUB)'''<br />
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{{Structure
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|PDB= 1sgv |SIZE=350|CAPTION= <scene name='initialview01'>1sgv</scene>, resolution 1.90&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70]
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|GENE= TRUB, RV2793C, MT2862.1, MTV002.58C, MB2816C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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}}
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'''STRUCTURE OF TRNA PSI55 PSEUDOURIDINE SYNTHASE (TRUB)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1SGV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry 1S71. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SGV OCA].
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1SGV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry 1S71. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SGV OCA].
==Reference==
==Reference==
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Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft., Chaudhuri BN, Chan S, Perry LJ, Yeates TO, J Biol Chem. 2004 Jun 4;279(23):24585-91. Epub 2004 Mar 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15028724 15028724]
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Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft., Chaudhuri BN, Chan S, Perry LJ, Yeates TO, J Biol Chem. 2004 Jun 4;279(23):24585-91. Epub 2004 Mar 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15028724 15028724]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Pseudouridylate synthase]]
[[Category: Pseudouridylate synthase]]
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[[Category: protein structure initiative]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: tb structural genomics consortium]]
[[Category: tb structural genomics consortium]]
[[Category: tbsgc]]
[[Category: tbsgc]]
[[Category: trna modification]]
[[Category: trna modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:04:44 2008''

Revision as of 12:04, 20 March 2008

Image:1sgv.gif


PDB ID 1sgv

Drag the structure with the mouse to rotate
, resolution 1.90Å
Gene: TRUB, RV2793C, MT2862.1, MTV002.58C, MB2816C (Mycobacterium tuberculosis)
Activity: Pseudouridylate synthase, with EC number 4.2.1.70
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF TRNA PSI55 PSEUDOURIDINE SYNTHASE (TRUB)


Overview

The three-dimensional structure of the RNA-modifying enzyme, psi55 tRNA pseudouridine synthase from Mycobacterium tuberculosis, is reported. The 1.9-A resolution crystal structure reveals the enzyme, free of substrate, in two distinct conformations. The structure depicts an interesting mode of protein flexibility involving a hinged bending in the central beta-sheet of the catalytic module. Key parts of the active site cleft are also found to be disordered in the substrate-free form of the enzyme. The hinge bending appears to act as a clamp to position the substrate. Our structural data furthers the previously proposed mechanism of tRNA recognition. The present crystal structure emphasizes the significant role that protein dynamics must play in tRNA recognition, base flipping, and modification.

About this Structure

1SGV is a Single protein structure of sequence from Mycobacterium tuberculosis. This structure supersedes the now removed PDB entry 1S71. Full crystallographic information is available from OCA.

Reference

Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft., Chaudhuri BN, Chan S, Perry LJ, Yeates TO, J Biol Chem. 2004 Jun 4;279(23):24585-91. Epub 2004 Mar 17. PMID:15028724

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