1shk

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[[Image:1shk.gif|left|200px]]<br /><applet load="1shk" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1shk.gif|left|200px]]
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caption="1shk, resolution 1.9&Aring;" />
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'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI'''<br />
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{{Structure
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|PDB= 1shk |SIZE=350|CAPTION= <scene name='initialview01'>1shk</scene>, resolution 1.9&Aring;
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|SITE= <scene name='pdbsite=PIP:A+Walker+Type+A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>PIP</scene>, <scene name='pdbsite=POP:A+Walker+Type+A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>POP</scene>, <scene name='pdbsite=SAS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SAS</scene> and <scene name='pdbsite=SBS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SBS</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71]
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|GENE= AROL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])
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}}
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'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1SHK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Shikimate_kinase Shikimate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71] Known structural/functional Sites: <scene name='pdbsite=PIP:A+Walker+Type+A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>PIP</scene>, <scene name='pdbsite=POP:A+Walker+Type+A+Motif+Forms+The+P-Loop+Which+Is+The+Bind+...'>POP</scene>, <scene name='pdbsite=SAS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SAS</scene> and <scene name='pdbsite=SBS:The+Electron+Density+For+Shikimate+Was+Ambiguous+Prevent+...'>SBS</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHK OCA].
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1SHK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHK OCA].
==Reference==
==Reference==
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Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299895 15299895]
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Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299895 15299895]
[[Category: Erwinia chrysanthemi]]
[[Category: Erwinia chrysanthemi]]
[[Category: Shikimate kinase]]
[[Category: Shikimate kinase]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:05:02 2008''

Revision as of 12:05, 20 March 2008

Image:1shk.gif


PDB ID 1shk

Drag the structure with the mouse to rotate
, resolution 1.9Å
Sites: , , and
Ligands:
Gene: AROL (Erwinia chrysanthemi)
Activity: Shikimate kinase, with EC number 2.7.1.71
Coordinates: save as pdb, mmCIF, xml



THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI


Overview

Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).

About this Structure

1SHK is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:15299895

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