4kys
From Proteopedia
(Difference between revisions)
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| - | + | ==Clostridium botulinum thiaminase I in complex with thiamin== | |
| - | ===Clostridium botulinum thiaminase I | + | <StructureSection load='4kys' size='340' side='right' caption='[[4kys]], [[Resolution|resolution]] 2.18Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4kys]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clob1 Clob1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KYS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KYS FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VIB:3-(4-AMINO-2-METHYL-PYRIMIDIN-5-YLMETHYL)-5-(2-HYDROXY-ETHYL)-4-METHYL-THIAZOL-3-IUM'>VIB</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CLB_0812 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=441770 CLOB1])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine_pyridinylase Thiamine pyridinylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.2 2.5.1.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kys OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kys RCSB], [http://www.ebi.ac.uk/pdbsum/4kys PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Thiaminases are responsible for the degradation of thiamin and its metabolites. Two classes of thiaminases have been identified based on their three-dimensional structures and in their requirements for a nucleophilic second substrate. While the reactions of several thiaminases have been characterized, the physiological role of thiamin degradation is not fully understood. We have determined the three-dimensional X-ray structure of an inactive C143S mutant of Clostridium botulinum (Cb) thiaminase I with bound thiamin at 2.2 A resolution. The C143S/thiamin complex provides atomic level details of the orientation of thiamin upon binding to Cb-thiaminase I and the identity of active site residues involved in substrate binding and catalysis. The specific roles of active site residues were probed using site directed mutagenesis and kinetic analyses, leading to a detailed mechanism for Cb-thiaminase I. The structure of Cb-thiaminase I is also compared to the functionally similar but structurally distinct thiaminase II. | ||
| - | + | Structure of a Clostridium botulinum C143S thiaminase I/thiamin complex reveals active site architecture.,Sikowitz MD, Shome B, Begley TP, Ealick SE Biochemistry. 2013 Sep 30. PMID:24079939<ref>PMID:24079939</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Clob1]] | [[Category: Clob1]] | ||
[[Category: Thiamine pyridinylase]] | [[Category: Thiamine pyridinylase]] | ||
| - | [[Category: Begley, T P | + | [[Category: Begley, T P]] |
| - | [[Category: Ealick, S E | + | [[Category: Ealick, S E]] |
| - | [[Category: Shome, B | + | [[Category: Shome, B]] |
| - | [[Category: Sikowitz, M D | + | [[Category: Sikowitz, M D]] |
| - | [[Category: Zhang, Y | + | [[Category: Zhang, Y]] |
[[Category: Periplasmic binding protein type 2 fold]] | [[Category: Periplasmic binding protein type 2 fold]] | ||
[[Category: Thiamin degradation]] | [[Category: Thiamin degradation]] | ||
[[Category: Thiaminase i]] | [[Category: Thiaminase i]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 15:29, 21 December 2014
Clostridium botulinum thiaminase I in complex with thiamin
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