This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1sip
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1sip.gif|left|200px]] | + | [[Image:1sip.gif|left|200px]] |
| - | + | ||
| - | '''ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE''' | + | {{Structure |
| + | |PDB= 1sip |SIZE=350|CAPTION= <scene name='initialview01'>1sip</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1SIP is a [ | + | 1SIP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIP OCA]. |
==Reference== | ==Reference== | ||
| - | Alternative native flap conformation revealed by 2.3 A resolution structure of SIV proteinase., Wilderspin AF, Sugrue RJ, J Mol Biol. 1994 May 27;239(1):97-103. PMID:[http:// | + | Alternative native flap conformation revealed by 2.3 A resolution structure of SIV proteinase., Wilderspin AF, Sugrue RJ, J Mol Biol. 1994 May 27;239(1):97-103. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8196050 8196050] |
[[Category: Simian immunodeficiency virus]] | [[Category: Simian immunodeficiency virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 16: | Line 25: | ||
[[Category: hydrolase(acid proteinase)]] | [[Category: hydrolase(acid proteinase)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:05:25 2008'' |
Revision as of 12:05, 20 March 2008
| |||||||
| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE
Overview
A large conformational change is observed between HIV-1 proteinase in the ligand-free state and in complexes with transition-state inhibitors. Crystal structures of this enzyme have either the flaps open for the native or ligand-free enzyme or the flaps closed for peptidomimetic ligand-bound enzyme. We describe the structure of native recombinant SIV proteinase which like other retroviral proteinases crystallizes as a perfect 2-fold symmetric dimer but in a different crystal packing arrangement. In contrast to HIV-1 PR we show that SIV proteinase in the ligand-free state adopts the closed flaps conformation, demonstrating that ligand binding is not a prerequisite for the closed flaps conformation. The catalytic water was clearly observed between the two aspartates which were not perfectly co-planar, and in this structure the active site cleft is more restricted than for either inhibitor bound or ligand-free HIV-1 proteinase. Accommodation of two bulkier side-chains in the simian enzyme core has resulted in a more exposed N terminus than for HIV-1 PR which we predict could enhance autocatalytic cleavage at the N terminus.
About this Structure
1SIP is a Single protein structure of sequence from Simian immunodeficiency virus. Full crystallographic information is available from OCA.
Reference
Alternative native flap conformation revealed by 2.3 A resolution structure of SIV proteinase., Wilderspin AF, Sugrue RJ, J Mol Biol. 1994 May 27;239(1):97-103. PMID:8196050
Page seeded by OCA on Thu Mar 20 14:05:25 2008
