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1sjm

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[[Image:1sjm.gif|left|200px]]<br /><applet load="1sjm" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1sjm.gif|left|200px]]
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caption="1sjm, resolution 1.40&Aring;" />
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'''Nitrite bound copper containing nitrite reductase'''<br />
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{{Structure
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|PDB= 1sjm |SIZE=350|CAPTION= <scene name='initialview01'>1sjm</scene>, resolution 1.40&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1]
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|GENE= NIRK, NIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511 Alcaligenes faecalis])
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}}
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'''Nitrite bound copper containing nitrite reductase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1SJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=NO2:'>NO2</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJM OCA].
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1SJM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJM OCA].
==Reference==
==Reference==
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Side-on copper-nitrosyl coordination by nitrite reductase., Tocheva EI, Rosell FI, Mauk AG, Murphy ME, Science. 2004 May 7;304(5672):867-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15131305 15131305]
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Side-on copper-nitrosyl coordination by nitrite reductase., Tocheva EI, Rosell FI, Mauk AG, Murphy ME, Science. 2004 May 7;304(5672):867-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15131305 15131305]
[[Category: Alcaligenes faecalis]]
[[Category: Alcaligenes faecalis]]
[[Category: Nitrite reductase (NO-forming)]]
[[Category: Nitrite reductase (NO-forming)]]
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[[Category: nitrite]]
[[Category: nitrite]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:05:48 2008''

Revision as of 12:05, 20 March 2008

Image:1sjm.gif


PDB ID 1sjm

Drag the structure with the mouse to rotate
, resolution 1.40Å
Ligands: , , and
Gene: NIRK, NIR (Alcaligenes faecalis)
Activity: Nitrite reductase (NO-forming), with EC number 1.7.2.1
Coordinates: save as pdb, mmCIF, xml



Nitrite bound copper containing nitrite reductase


Overview

A copper-nitrosyl intermediate forms during the catalytic cycle of nitrite reductase, the enzyme that mediates the committed step in bacterial denitrification. The crystal structure of a type 2 copper-nitrosyl complex of nitrite reductase reveals an unprecedented side-on binding mode in which the nitrogen and oxygen atoms are nearly equidistant from the copper cofactor. Comparison of this structure with a refined nitrite-bound crystal structure explains how coordination can change between copper-oxygen and copper-nitrogen during catalysis. The side-on copper-nitrosyl in nitrite reductase expands the possibilities for nitric oxide interactions in copper proteins such as superoxide dismutase and prions.

About this Structure

1SJM is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

Reference

Side-on copper-nitrosyl coordination by nitrite reductase., Tocheva EI, Rosell FI, Mauk AG, Murphy ME, Science. 2004 May 7;304(5672):867-70. PMID:15131305

Page seeded by OCA on Thu Mar 20 14:05:48 2008

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