4j2n
From Proteopedia
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| - | + | ==Crystal Structure of mycobacteriophage Pukovnik Xis== | |
| - | === | + | <StructureSection load='4j2n' size='340' side='right' caption='[[4j2n]], [[Resolution|resolution]] 2.35Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4j2n]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacteriophage_pukovnik Mycobacteriophage pukovnik]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J2N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J2N FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">37, Pukovnik_37, XIS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=540068 Mycobacteriophage Pukovnik])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j2n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j2n RCSB], [http://www.ebi.ac.uk/pdbsum/4j2n PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The recombination directionality factor, Xis, is a DNA bending protein that determines the outcome of integrase-mediated site-specific recombination by redesign of higher-order protein-DNA architectures. Although the attachment site DNA of mycobacteriophage Pukovnik is likely to contain four sites for Xis binding, Xis crystals contain five subunits in the asymmetric unit, four of which align into a Xis filament and a fifth that is generated by an unusual domain swap. Extensive intersubunit contacts stabilize a bent filament-like arrangement with Xis monomers aligned head to tail. The structure implies a DNA bend of ~120 degrees , which is in agreement with DNA bending measured in vitro. Formation of attR-containing intasomes requires only Int and Xis, distinguishing Pukovnik from lambda. Therefore, we conclude that, in Pukovnik, Xis-induced DNA bending is sufficient to promote intramolecular Int-mediated bridges during intasome formation. | ||
| - | + | The Structure of Xis Reveals the Basis for Filament Formation and Insight into DNA Bending within a Mycobacteriophage Intasome.,Singh S, Plaks JG, Homa NJ, Amrich CG, Heroux A, Hatfull GF, Vandemark AP J Mol Biol. 2013 Oct 7. pii: S0022-2836(13)00628-1. doi:, 10.1016/j.jmb.2013.10.002. PMID:24112940<ref>PMID:24112940</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Mycobacteriophage pukovnik]] | [[Category: Mycobacteriophage pukovnik]] | ||
| - | [[Category: Amrich, C G | + | [[Category: Amrich, C G]] |
| - | [[Category: Heroux, A | + | [[Category: Heroux, A]] |
| - | [[Category: Homa, N J | + | [[Category: Homa, N J]] |
| - | [[Category: VanDemark, A P | + | [[Category: VanDemark, A P]] |
[[Category: Doman swap]] | [[Category: Doman swap]] | ||
[[Category: Filament]] | [[Category: Filament]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
[[Category: Winged-helix]] | [[Category: Winged-helix]] | ||
Revision as of 16:13, 21 December 2014
Crystal Structure of mycobacteriophage Pukovnik Xis
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