3ai4

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[[Image:3ai4.png|left|200px]]
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==Crystal structure of yeast enhanced green fluorescent protein - mouse polymerase iota ubiquitin binding motif fusion protein==
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<StructureSection load='3ai4' size='340' side='right' caption='[[3ai4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ai4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AI4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AI4 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ai5|3ai5]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ai4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ai4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ai4 RCSB], [http://www.ebi.ac.uk/pdbsum/3ai4 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ai/3ai4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The generation of crystal lattice contacts by proteinaceous tags fused to target proteins is an attractive approach to aid in the crystallization of otherwise intractable proteins. Here, the use of green fluorescent protein (GFP) fusions for this purpose is demonstrated, using ubiquitin and the ubiquitin-binding motif (UBM) of Y-family polymerase iota as examples. The structure of the GFP-ubiquitin fusion protein revealed that the crystal lattice was formed by GFP moieties. Ubiquitin was accommodated in the lattice through interactions with the peripheral loops of GFP. However, in the GFP-UBM fusion crystal UBM formed extensive interactions with GFP and these interactions, together with UBM dimerization, mediated the crystal packing. Interestingly, the tyrosine residues that are involved in mediating crystal contacts in both GFP-ubiquitin and GFP-UBM crystals are arranged in a belt on the surface of the beta-barrel structure of GFP. Therefore, it is likely that GFP can assist in the crystallization of small proteins and of protein domains in general.
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{{STRUCTURE_3ai4| PDB=3ai4 | SCENE= }}
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Crystallization of small proteins assisted by green fluorescent protein.,Suzuki N, Hiraki M, Yamada Y, Matsugaki N, Igarashi N, Kato R, Dikic I, Drew D, Iwata S, Wakatsuki S, Kawasaki M Acta Crystallogr D Biol Crystallogr. 2010 Oct;66(Pt 10):1059-66. Epub 2010, Sep 18. PMID:20944239<ref>PMID:20944239</ref>
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===Crystal structure of yeast enhanced green fluorescent protein - mouse polymerase iota ubiquitin binding motif fusion protein===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_20944239}}
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==About this Structure==
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[[3ai4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AI4 OCA].
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==See Also==
==See Also==
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*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
 
*[[DNA polymerase|DNA polymerase]]
*[[DNA polymerase|DNA polymerase]]
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*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
 
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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*[[Sandbox104|Sandbox104]]
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== References ==
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*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:020944239</ref><references group="xtra"/>
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Kawasaki, M.]]
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[[Category: Kawasaki, M]]
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[[Category: Suzuki, N.]]
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[[Category: Suzuki, N]]
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[[Category: Wakatsuki, S.]]
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[[Category: Wakatsuki, S]]
[[Category: Fluorescent protein]]
[[Category: Fluorescent protein]]
[[Category: Fusion]]
[[Category: Fusion]]

Revision as of 16:18, 21 December 2014

Crystal structure of yeast enhanced green fluorescent protein - mouse polymerase iota ubiquitin binding motif fusion protein

3ai4, resolution 1.60Å

Drag the structure with the mouse to rotate

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