1smg
From Proteopedia
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| - | [[Image:1smg.gif|left|200px]] | + | [[Image:1smg.gif|left|200px]] |
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| - | '''CALCIUM-BOUND E41A MUTANT OF THE N-DOMAIN OF CHICKEN TROPONIN C, NMR, 40 STRUCTURES''' | + | {{Structure |
| + | |PDB= 1smg |SIZE=350|CAPTION= <scene name='initialview01'>1smg</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CALCIUM-BOUND E41A MUTANT OF THE N-DOMAIN OF CHICKEN TROPONIN C, NMR, 40 STRUCTURES''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SMG is a [ | + | 1SMG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMG OCA]. |
==Reference== | ==Reference== | ||
| - | Mechanism of direct coupling between binding and induced structural change in regulatory calcium binding proteins., Gagne SM, Li MX, Sykes BD, Biochemistry. 1997 Apr 15;36(15):4386-92. PMID:[http:// | + | Mechanism of direct coupling between binding and induced structural change in regulatory calcium binding proteins., Gagne SM, Li MX, Sykes BD, Biochemistry. 1997 Apr 15;36(15):4386-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9109645 9109645] |
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: troponin c]] | [[Category: troponin c]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:06:53 2008'' |
Revision as of 12:06, 20 March 2008
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CALCIUM-BOUND E41A MUTANT OF THE N-DOMAIN OF CHICKEN TROPONIN C, NMR, 40 STRUCTURES
Overview
The structural transition in troponin C induced by the binding of two calcium ions involves an "opening" of the structure, an event that triggers skeletal muscle contraction. We have solved the solution structure of a mutant (E41A) of the regulatory domain of skeletal troponin C wherein one bidentate ligand to the calcium in site I is missing. This structure remains "closed" upon calcium binding, indicating that the linkage between calcium binding and the induced conformational change has been broken. This provides a snapshot of skeletal troponin C between the off and on state and thereby valuable insight into the mechanism of regulation within skeletal TnC. Although several factors contribute to the triggering mechanism, the opening of the troponin C structure is ultimately dependent on one amino acid, Glu41. Insights into the structure of cardiac troponin C can also be derived from this skeletal mutant.
About this Structure
1SMG is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Mechanism of direct coupling between binding and induced structural change in regulatory calcium binding proteins., Gagne SM, Li MX, Sykes BD, Biochemistry. 1997 Apr 15;36(15):4386-92. PMID:9109645
Page seeded by OCA on Thu Mar 20 14:06:53 2008
