1smn
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1smn.gif|left|200px]] | + | [[Image:1smn.gif|left|200px]] |
| - | + | ||
| - | '''IDENTIFICATION OF THE SERRATIA ENDONUCLEASE DIMER: STRUCTURAL BASIS AND IMPLICATIONS FOR CATALYSIS''' | + | {{Structure |
| + | |PDB= 1smn |SIZE=350|CAPTION= <scene name='initialview01'>1smn</scene>, resolution 2.04Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Serratia_marcescens_nuclease Serratia marcescens nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.30.2 3.1.30.2] | ||
| + | |GENE= NUCA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=615 Serratia marcescens]) | ||
| + | }} | ||
| + | |||
| + | '''IDENTIFICATION OF THE SERRATIA ENDONUCLEASE DIMER: STRUCTURAL BASIS AND IMPLICATIONS FOR CATALYSIS''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1SMN is a [ | + | 1SMN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMN OCA]. |
==Reference== | ==Reference== | ||
| - | Identification of the Serratia endonuclease dimer: structural basis and implications for catalysis., Miller MD, Krause KL, Protein Sci. 1996 Jan;5(1):24-33. PMID:[http:// | + | Identification of the Serratia endonuclease dimer: structural basis and implications for catalysis., Miller MD, Krause KL, Protein Sci. 1996 Jan;5(1):24-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8771193 8771193] |
[[Category: Serratia marcescens]] | [[Category: Serratia marcescens]] | ||
[[Category: Serratia marcescens nuclease]] | [[Category: Serratia marcescens nuclease]] | ||
| Line 21: | Line 30: | ||
[[Category: sugar-nonspecific nuclease]] | [[Category: sugar-nonspecific nuclease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:06:58 2008'' |
Revision as of 12:07, 20 March 2008
| |||||||
| , resolution 2.04Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | NUCA (Serratia marcescens) | ||||||
| Activity: | Serratia marcescens nuclease, with EC number 3.1.30.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
IDENTIFICATION OF THE SERRATIA ENDONUCLEASE DIMER: STRUCTURAL BASIS AND IMPLICATIONS FOR CATALYSIS
Overview
The Serratia endonuclease is an extracellularly secreted enzyme capable of cleaving both single- and double-stranded forms of DNA and RNA. It is the first member of a large class of related and usually dimeric endonucleases for which a structure is known. Using X-ray crystallography, the structure of monomer of this enzyme was reported by us previously (Miller MD et al., 1994, Nature Struct Biol 1:461-468). We now confirm the dimeric nature of this enzyme through light-scattering experiments and identify the physiologic dimer interface through crystal packing analysis. This dimerization occurs through an isologous twofold interaction localized to the carboxy-terminal subdomain of the enzyme. The dimer is a prolate ellipsoid with dimensions 30 A x 35 A x 90 A. The dimer interface is flat and contains four salt links, several hydrogen bonds, and nonpolar interactions. Buried water is prominent in this interface and it includes an unusual "cubic" water cluster. The position of the two active sites in the dimer suggests that they can act independently in their cleavage of DNA, but have a geometrical advantage in attacking substrate relative to the monomer.
About this Structure
1SMN is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.
Reference
Identification of the Serratia endonuclease dimer: structural basis and implications for catalysis., Miller MD, Krause KL, Protein Sci. 1996 Jan;5(1):24-33. PMID:8771193
Page seeded by OCA on Thu Mar 20 14:06:58 2008
