4nec
From Proteopedia
(Difference between revisions)
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| - | + | ==Conversion of a Disulfide Bond into a Thioacetal Group during Echinomycin Biosynthesis== | |
| - | + | <StructureSection load='4nec' size='340' side='right' caption='[[4nec]], [[Resolution|resolution]] 1.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4nec]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/'streptomyces_lasaliensis' 'streptomyces lasaliensis']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NEC FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=QUI:2-CARBOXYQUINOXALINE'>QUI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSN:D-SERINE'>DSN</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=N2C:N,S-DIMETHYLCYSTEINE'>N2C</scene>, <scene name='pdbligand=NCY:N-METHYLCYSTEINE'>NCY</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ecm18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=324833 'Streptomyces lasaliensis'])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nec OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nec RCSB], [http://www.ebi.ac.uk/pdbsum/4nec PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Echinomycin is a nonribosomal depsipeptide natural product with a range of interesting bioactivities that make it an important target for drug discovery and development. It contains a thioacetal bridge, a unique chemical motif derived from the disulfide bond of its precursor antibiotic triostin A by the action of an S-adenosyl-L-methionine-dependent methyltransferase, Ecm18. The crystal structure of Ecm18 in complex with its reaction products S-adenosyl-L-homocysteine and echinomycin was determined at 1.50 A resolution. Phasing was achieved using a new molecular replacement package called AMPLE, which automatically derives search models from structure predictions based on ab initio protein modelling. Structural analysis indicates that a combination of proximity effects, medium effects, and catalysis by strain drives the unique transformation of the disulfide bond into the thioacetal linkage. | ||
| - | + | Conversion of a Disulfide Bond into a Thioacetal Group during Echinomycin Biosynthesis.,Hotta K, Keegan RM, Ranganathan S, Fang M, Bibby J, Winn MD, Sato M, Lian M, Watanabe K, Rigden DJ, Kim CY Angew Chem Int Ed Engl. 2013 Dec 2. doi: 10.1002/anie.201307404. PMID:24302672<ref>PMID:24302672</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Bibby, J | + | == References == |
| - | [[Category: Fang, M | + | <references/> |
| - | [[Category: Hotta, K | + | __TOC__ |
| - | [[Category: Keegan, R M | + | </StructureSection> |
| - | [[Category: Kim, C Y | + | [[Category: Streptomyces lasaliensis]] |
| - | [[Category: Lian, M | + | [[Category: Bibby, J]] |
| - | [[Category: Ranganathan, S | + | [[Category: Fang, M]] |
| - | [[Category: Rigden, D J | + | [[Category: Hotta, K]] |
| - | [[Category: Sato, M | + | [[Category: Keegan, R M]] |
| - | [[Category: Watanabe, K | + | [[Category: Kim, C Y]] |
| - | [[Category: Winn, M D | + | [[Category: Lian, M]] |
| + | [[Category: Ranganathan, S]] | ||
| + | [[Category: Rigden, D J]] | ||
| + | [[Category: Sato, M]] | ||
| + | [[Category: Watanabe, K]] | ||
| + | [[Category: Winn, M D]] | ||
[[Category: Methyltransferase]] | [[Category: Methyltransferase]] | ||
[[Category: Sam-dependent methyltransferase]] | [[Category: Sam-dependent methyltransferase]] | ||
[[Category: Transferase-antibiotic complex]] | [[Category: Transferase-antibiotic complex]] | ||
Revision as of 16:43, 21 December 2014
Conversion of a Disulfide Bond into a Thioacetal Group during Echinomycin Biosynthesis
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