4mx3
From Proteopedia
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| - | + | ==Crystal Structure of PKA RIalpha Homodimer== | |
| - | === | + | <StructureSection load='4mx3' size='340' side='right' caption='[[4mx3]], [[Resolution|resolution]] 3.88Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4mx3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MX3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rgs|1rgs]], [[1ne4|1ne4]], [[1ne6|1ne6]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRKAR1A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4mx3 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The regulatory (R) subunit is the cAMP receptor of protein kinase A. Following cAMP binding, the inactive PKA holoenzyme complex separates into two active catalytic (C) subunits and a cAMP-bound R dimer. Thus far, only monomeric R structures have been solved, which fell short in explaining differences of cAMP binding for the full-length protein as compared to the truncated R subunits. Here we solved a full-length R-dimer structure that reflects the biologically relevant conformation, and this structure agrees well with small angle X-ray scattering. An isoform-specific interface is revealed between the protomers. This interface acts as an intermolecular sensor for cAMP and explains the cooperative character of cAMP binding to the RIalpha dimer. Mutagenesis of residues on this interface not only leads to structural and biochemical changes, but is also linked to Carney complex disease. | ||
| - | + | PKA RIalpha Homodimer Structure Reveals an Intermolecular Interface with Implications for Cooperative cAMP Binding and Carney Complex Disease.,Bruystens JG, Wu J, Fortezzo A, Kornev AP, Blumenthal DK, Taylor SS Structure. 2014 Jan 7;22(1):59-69. doi: 10.1016/j.str.2013.10.012. Epub 2013 Dec , 5. PMID:24316401<ref>PMID:24316401</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| - | [[Category: Blumenthal, D A | + | </StructureSection> |
| - | [[Category: Bruystens, J G.H | + | [[Category: Bovin]] |
| - | [[Category: Fortezzo, A | + | [[Category: Blumenthal, D A]] |
| - | [[Category: Kornev, A P | + | [[Category: Bruystens, J G.H]] |
| - | [[Category: Taylor, S S | + | [[Category: Fortezzo, A]] |
| - | [[Category: Wu, J | + | [[Category: Kornev, A P]] |
| + | [[Category: Taylor, S S]] | ||
| + | [[Category: Wu, J]] | ||
[[Category: Carney complex disease]] | [[Category: Carney complex disease]] | ||
[[Category: Cooperative camp binding]] | [[Category: Cooperative camp binding]] | ||
Revision as of 16:49, 21 December 2014
Crystal Structure of PKA RIalpha Homodimer
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