1stf
From Proteopedia
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| - | [[Image:1stf.gif|left|200px]] | + | [[Image:1stf.gif|left|200px]] |
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| - | '''THE REFINED 2.4 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF RECOMBINANT HUMAN STEFIN B IN COMPLEX WITH THE CYSTEINE PROTEINASE PAPAIN: A NOVEL TYPE OF PROTEINASE INHIBITOR INTERACTION''' | + | {{Structure |
| + | |PDB= 1stf |SIZE=350|CAPTION= <scene name='initialview01'>1stf</scene>, resolution 2.37Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE REFINED 2.4 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF RECOMBINANT HUMAN STEFIN B IN COMPLEX WITH THE CYSTEINE PROTEINASE PAPAIN: A NOVEL TYPE OF PROTEINASE INHIBITOR INTERACTION''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1STF is a [ | + | 1STF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STF OCA]. |
==Reference== | ==Reference== | ||
| - | The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction., Stubbs MT, Laber B, Bode W, Huber R, Jerala R, Lenarcic B, Turk V, EMBO J. 1990 Jun;9(6):1939-47. PMID:[http:// | + | The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction., Stubbs MT, Laber B, Bode W, Huber R, Jerala R, Lenarcic B, Turk V, EMBO J. 1990 Jun;9(6):1939-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2347312 2347312] |
[[Category: Carica papaya]] | [[Category: Carica papaya]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: hydrolase(sulfhydryl proteinase)]] | [[Category: hydrolase(sulfhydryl proteinase)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:09:25 2008'' |
Revision as of 12:09, 20 March 2008
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| , resolution 2.37Å | |||||||
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| Activity: | Papain, with EC number 3.4.22.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE REFINED 2.4 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF RECOMBINANT HUMAN STEFIN B IN COMPLEX WITH THE CYSTEINE PROTEINASE PAPAIN: A NOVEL TYPE OF PROTEINASE INHIBITOR INTERACTION
Contents |
Overview
A stoichiometric complex of human stefin B and carboxymethylated papain has been crystallized in a trigonal crystal form. Data to 2.37 A resolution were collected using the area detector diffractometer FAST. The crystal structure of the complex has been solved by Patterson search techniques using papain as search model. Starting from the structure of chicken cystatin, the stefin structure was elucidated through cycles of model building and crystallographic refinement. The current crystallographic R factor is 0.19. Like cystatin, the stefin molecule consists of a five stranded beta-sheet wrapped around a five turn alpha-helix, but with an additional carboxy terminal strand running along the convex side of the sheet. Topological equivalence of stefin and cystatin reveal the previous sequence alignment to be incorrect in part, through deletion of the intermediate helix. The conserved residues form a tripartite wedge, which slots into the papain active site as proposed through consideration of the tertiary structures of the individual components (Bode et al., 1988). The main interactions are provided by the amino terminal 'trunk' (occupying the 'unprimed' subsites of the enzyme), and by the first hairpin loop, containing the highly conserved QVVAG sequence, with minor contributions from the second hairpin loop. The carboxyl terminus of stefin provides an additional interaction region with respect to cystatin. The interaction is dominated by hydrophobic contacts. Inhibition by the cysteine proteinase inhibitors is fundamentally different to that observed for the serine proteinase inhibitors.
Disease
Known diseases associated with this structure: Epilepsy, progressive myoclonic 1 OMIM:[601145]
About this Structure
1STF is a Protein complex structure of sequences from Carica papaya and Homo sapiens. Full crystallographic information is available from OCA.
Reference
The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction., Stubbs MT, Laber B, Bode W, Huber R, Jerala R, Lenarcic B, Turk V, EMBO J. 1990 Jun;9(6):1939-47. PMID:2347312
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