1svx
From Proteopedia
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| - | [[Image:1svx.gif|left|200px]] | + | [[Image:1svx.gif|left|200px]] |
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| - | '''Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein''' | + | {{Structure |
| + | |PDB= 1svx |SIZE=350|CAPTION= <scene name='initialview01'>1svx</scene>, resolution 2.24Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= MALE, B4034, Z5632, ECS5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SVX is a [ | + | 1SVX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SVX OCA]. |
==Reference== | ==Reference== | ||
| - | High-affinity binders selected from designed ankyrin repeat protein libraries., Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter MG, Pluckthun A, Nat Biotechnol. 2004 May;22(5):575-82. Epub 2004 Apr 18. PMID:[http:// | + | High-affinity binders selected from designed ankyrin repeat protein libraries., Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter MG, Pluckthun A, Nat Biotechnol. 2004 May;22(5):575-82. Epub 2004 Apr 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15097997 15097997] |
[[Category: ]] | [[Category: ]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: selected binder]] | [[Category: selected binder]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:10:23 2008'' |
Revision as of 12:10, 20 March 2008
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| , resolution 2.24Å | |||||||
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| Gene: | MALE, B4034, Z5632, ECS5017 (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein
Overview
We report here the evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity target binding. Using a consensus design strategy, we generated combinatorial libraries of AR proteins of varying repeat numbers with diversified binding surfaces. Libraries of two and three repeats, flanked by 'capping repeats,' were used in ribosome-display selections against maltose binding protein (MBP) and two eukaryotic kinases. We rapidly enriched target-specific binders with affinities in the low nanomolar range and determined the crystal structure of one of the selected AR proteins in complex with MBP at 2.3 A resolution. The interaction relies on the randomized positions of the designed AR protein and is comparable to natural, heterodimeric protein-protein interactions. Thus, our AR protein libraries are valuable sources for binding molecules and, because of the very favorable biophysical properties of the designed AR proteins, an attractive alternative to antibody libraries.
About this Structure
1SVX is a Protein complex structure of sequences from [1] and Escherichia coli. Full crystallographic information is available from OCA.
Reference
High-affinity binders selected from designed ankyrin repeat protein libraries., Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter MG, Pluckthun A, Nat Biotechnol. 2004 May;22(5):575-82. Epub 2004 Apr 18. PMID:15097997 [[Category: ]]
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