1swi

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[[Image:1swi.gif|left|200px]]<br /><applet load="1swi" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1swi.gif|left|200px]]
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caption="1swi, resolution 2.6&Aring;" />
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'''GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE'''<br />
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{{Structure
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|PDB= 1swi |SIZE=350|CAPTION= <scene name='initialview01'>1swi</scene>, resolution 2.6&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=BNZ:BENZENE'>BNZ</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1SWI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=BNZ:'>BNZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWI OCA].
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1SWI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWI OCA].
==Reference==
==Reference==
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An engineered allosteric switch in leucine-zipper oligomerization., Gonzalez L Jr, Plecs JJ, Alber T, Nat Struct Biol. 1996 Jun;3(6):510-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8646536 8646536]
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An engineered allosteric switch in leucine-zipper oligomerization., Gonzalez L Jr, Plecs JJ, Alber T, Nat Struct Biol. 1996 Jun;3(6):510-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8646536 8646536]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: peptide]]
[[Category: peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:06:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:10:37 2008''

Revision as of 12:10, 20 March 2008

Image:1swi.gif


PDB ID 1swi

Drag the structure with the mouse to rotate
, resolution 2.6Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE


Overview

Controversy remains about the role of core side-chain packing in specifying protein structure. To investigate the influence of core packing on the oligomeric structure of a coiled coil, we engineered a GCN4 leucine zipper mutant that switches from two to three strands upon binding the hydrophobic ligands cyclohexane and benzene. In solution these ligands increased the apparent thermal stability and the oligomerization order of the mutant leucine zipper. The crystal structure of the peptide-benzene complex shows a single benzene molecule bound at the engineered site in the core of the trimer. These results indicate that coiled coils are well-suited to function as molecular switches and emphasize that core packing is an important determinant of oligomerization specificity.

About this Structure

1SWI is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

An engineered allosteric switch in leucine-zipper oligomerization., Gonzalez L Jr, Plecs JJ, Alber T, Nat Struct Biol. 1996 Jun;3(6):510-5. PMID:8646536

Page seeded by OCA on Thu Mar 20 14:10:37 2008

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