1swu
From Proteopedia
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| - | [[Image:1swu.gif|left|200px]] | + | [[Image:1swu.gif|left|200px]] |
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| - | '''STREPTAVIDIN MUTANT Y43F''' | + | {{Structure |
| + | |PDB= 1swu |SIZE=350|CAPTION= <scene name='initialview01'>1swu</scene>, resolution 1.14Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene> and <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STREPTAVIDIN MUTANT Y43F''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SWU is a [ | + | 1SWU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. |
==Reference== | ==Reference== | ||
| - | Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site?, Freitag S, Le Trong I, Klumb LA, Stayton PS, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1118-26. PMID:[http:// | + | Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site?, Freitag S, Le Trong I, Klumb LA, Stayton PS, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1118-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10329773 10329773] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces avidinii]] | [[Category: Streptomyces avidinii]] | ||
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[[Category: protein binding]] | [[Category: protein binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:10:41 2008'' |
Revision as of 12:10, 20 March 2008
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| , resolution 1.14Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STREPTAVIDIN MUTANT Y43F
Overview
The streptavidin-biotin system is an example of a high-affinity protein-ligand pair (Ka approximately 10(13) mol-1). The thermodynamic and structural properties have been extensively studied as a model system for protein-ligand interactions. Here, the X-ray crystal structure of a streptavidin mutant of a residue hydrogen bonding to biotin [Tyr43Phe (Y43F)] is reported at atomic resolution (1.14 A). The biotin-free structure was refined with anisotropic displacement parameters (SHELXL97 program package). The high-resolution data also allowed interpretation of side-chain and residue disorder in 41 residues where alternate conformations were refined. The Y43F mutation is unambiguously observed in difference maps, although only a single O atom per monomer is altered. The atomic resolution enabled the identification of 2-methyl-2, 4-pentanediol (MPD) molecules in the biotin-binding pocket for the first time. Electron density for MPD was observed in all four subunit binding sites of the tetrameric protein. This was not possible with data at lower resolution (1.8-2.3 A) for wild-type streptavidin or mutants in the same crystal form using MPD in the crystallization. The impact of MPD binding on these studies is discussed.
About this Structure
1SWU is a Single protein structure of sequence from Streptomyces avidinii. Full crystallographic information is available from OCA.
Reference
Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site?, Freitag S, Le Trong I, Klumb LA, Stayton PS, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1118-26. PMID:10329773
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