1sxj
From Proteopedia
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| - | [[Image:1sxj.gif|left|200px]] | + | [[Image:1sxj.gif|left|200px]] |
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| - | '''Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)''' | + | {{Structure |
| + | |PDB= 1sxj |SIZE=350|CAPTION= <scene name='initialview01'>1sxj</scene>, resolution 2.85Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ATG:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>ATG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= RFC1, CDC44, YOR217W, YOR50-7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RFC4, YOL094C, O0923 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RFC3, YNL290W, N0533 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RFC2, YJR068W, J1808 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RFC5, YBR087W, YBR0810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), POL30, YBR088C, YBR0811 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SXJ is a [ | + | 1SXJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXJ OCA]. |
==Reference== | ==Reference== | ||
| - | Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex., Bowman GD, O'Donnell M, Kuriyan J, Nature. 2004 Jun 17;429(6993):724-30. PMID:[http:// | + | Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex., Bowman GD, O'Donnell M, Kuriyan J, Nature. 2004 Jun 17;429(6993):724-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15201901 15201901] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: processivity clamp]] | [[Category: processivity clamp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:10:56 2008'' |
Revision as of 12:10, 20 March 2008
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| , resolution 2.85Å | |||||||
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| Ligands: | , and | ||||||
| Gene: | RFC1, CDC44, YOR217W, YOR50-7 (Saccharomyces cerevisiae), RFC4, YOL094C, O0923 (Saccharomyces cerevisiae), RFC3, YNL290W, N0533 (Saccharomyces cerevisiae), RFC2, YJR068W, J1808 (Saccharomyces cerevisiae), RFC5, YBR087W, YBR0810 (Saccharomyces cerevisiae), POL30, YBR088C, YBR0811 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Eukaryotic Clamp Loader (Replication Factor C, RFC) Bound to the DNA Sliding Clamp (Proliferating Cell Nuclear Antigen, PCNA)
Overview
Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex (replication factor-C, RFC) of the yeast Saccharomyces cerevisiae, bound to the sliding clamp (proliferating cell nuclear antigen, PCNA). Tight interfacial coordination of the ATP analogue ATP-gammaS by RFC results in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring. Placement of a model for primed DNA within the central hole of PCNA reveals a striking correspondence between the RFC spiral and the grooves of the DNA double helix. This model, in which the clamp loader complex locks onto primed DNA in a screw-cap-like arrangement, provides a simple explanation for the process by which the engagement of primer-template junctions by the RFC:PCNA complex results in ATP hydrolysis and release of the sliding clamp on DNA.
About this Structure
1SXJ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex., Bowman GD, O'Donnell M, Kuriyan J, Nature. 2004 Jun 17;429(6993):724-30. PMID:15201901
Page seeded by OCA on Thu Mar 20 14:10:56 2008
