1sxy

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[[Image:1sxy.gif|left|200px]]<br /><applet load="1sxy" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1sxy.gif|left|200px]]
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caption="1sxy, resolution 1.07&Aring;" />
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'''1.07 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus'''<br />
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{{Structure
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|PDB= 1sxy |SIZE=350|CAPTION= <scene name='initialview01'>1sxy</scene>, resolution 1.07&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''1.07 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1SXY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus] with <scene name='pdbligand=NH4:'>NH4</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXY OCA].
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1SXY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXY OCA].
==Reference==
==Reference==
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Role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4., Maes EM, Weichsel A, Andersen JF, Shepley D, Montfort WR, Biochemistry. 2004 Jun 1;43(21):6679-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15157102 15157102]
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Role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4., Maes EM, Weichsel A, Andersen JF, Shepley D, Montfort WR, Biochemistry. 2004 Jun 1;43(21):6679-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15157102 15157102]
[[Category: Rhodnius prolixus]]
[[Category: Rhodnius prolixus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: lipocalin]]
[[Category: lipocalin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:06:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:11:05 2008''

Revision as of 12:11, 20 March 2008

Image:1sxy.gif


PDB ID 1sxy

Drag the structure with the mouse to rotate
, resolution 1.07Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



1.07 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus


Overview

Nitrophorins are ferric heme proteins that transport nitric oxide (NO) from blood-sucking insects to victims. NO binding is tighter at lower pH values, as found in the insect salivary gland, and weaker at the pH of the victim's tissue, facilitating NO release and subsequent vasodilation. Previous structural analyses of nitrophorin 4 (NP4) from Rhodnius prolixus revealed a substantial NO-induced conformational change involving the A-B and G-H loops, which rearrange to desolvate the distal pocket and pack nonpolar residues against the heme-ligated NO. Previous kinetic analyses revealed a slow, biphasic, and pH-dependent NO release, which was proposed to be associated with loop movements. In this study, we created NP4 mutants D30A and D30N (A-B loop), D129A/L130A (G-H loop), and T121V (distal pocket). Eight crystal structures were determined, including complexes with NO, NH(3), and imidazole, to resolutions as high as 1.0 A. The NO-induced conformational change is largely abolished in the loop mutants, but retained in T121V. Kinetic analyses using stopped-flow spectroscopy revealed the pH dependence for NO release is eliminated for D129A/L130A, considerably reduced for D30A and D30N, but retained for T121V. NO association rates were increased 2-5-fold for T121V, but were unchanged in the loop mutants. Taken together, our findings demonstrate that the pH dependency for NO release is linked to loop dynamics and that solvent reorganization is apparently rate-limiting for formation of the initial iron-nitrosyl bond. Interestingly, the multiphasic kinetic behavior of rNPs was not affected by mutations, and its cause remains unclear.

About this Structure

1SXY is a Single protein structure of sequence from Rhodnius prolixus. Full crystallographic information is available from OCA.

Reference

Role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4., Maes EM, Weichsel A, Andersen JF, Shepley D, Montfort WR, Biochemistry. 2004 Jun 1;43(21):6679-90. PMID:15157102

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