4myz
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Structure of a class 2 docking domain complex from modules CurK and CurL of the curacin A polyketide synthase== | |
| - | + | <StructureSection load='4myz' size='340' side='right' caption='[[4myz]], [[Resolution|resolution]] 1.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4myz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula_3l Lyngbya majuscula 3l]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MYZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4myy|4myy]], [[4mz0|4mz0]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LYNGBM3L_74450, LYNGBM3L_74440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489825 Lyngbya majuscula 3L])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4myz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4myz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4myz RCSB], [http://www.ebi.ac.uk/pdbsum/4myz PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Modular type I polyketide synthases (PKSs) are versatile biosynthetic systems that initiate, successively elongate, and modify acyl chains. Intermediate transfer between modules is mediated via docking domains, which are attractive targets for PKS pathway engineering to produce natural product analogs. We identified a class 2 docking domain in cyanobacterial PKSs and determined crystal structures for two docking domain pairs, revealing a distinct class 2 docking strategy for promoting intermediate transfer. The selectivity of class 2 docking interactions, demonstrated in binding and biochemical assays, could be altered by mutagenesis. We determined the ideal fusion location for exchanging class 1 and class 2 docking domains and demonstrated effective polyketide chain transfer in heterologous modules. Thus, class 2 docking domains are tools for rational bioengineering of a broad range of PKSs containing either class 1 or 2 docking domains. | ||
| - | + | Cyanobacterial polyketide synthase docking domains: a tool for engineering natural product biosynthesis.,Whicher JR, Smaga SS, Hansen DA, Brown WC, Gerwick WH, Sherman DH, Smith JL Chem Biol. 2013 Nov 21;20(11):1340-51. doi: 10.1016/j.chembiol.2013.09.015. Epub , 2013 Oct 31. PMID:24183970<ref>PMID:24183970</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Smaga, S S | + | == References == |
| - | [[Category: Smith, J L | + | <references/> |
| - | [[Category: Whicher, J R | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Lyngbya majuscula 3l]] | ||
| + | [[Category: Smaga, S S]] | ||
| + | [[Category: Smith, J L]] | ||
| + | [[Category: Whicher, J R]] | ||
[[Category: Fusion protein]] | [[Category: Fusion protein]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Protein-protein interaction]] | [[Category: Protein-protein interaction]] | ||
Revision as of 18:13, 21 December 2014
Structure of a class 2 docking domain complex from modules CurK and CurL of the curacin A polyketide synthase
| |||||||||||
