4ijr

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{{STRUCTURE_4ijr| PDB=4ijr | SCENE= }}
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==Crystal structure of Saccharomyces cerevisiae arabinose dehydrogenase Ara1 complexed with NADPH==
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===Crystal structure of Saccharomyces cerevisiae arabinose dehydrogenase Ara1 complexed with NADPH===
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<StructureSection load='4ijr' size='340' side='right' caption='[[4ijr]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24192347}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ijr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IJR FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ijc|4ijc]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARA1, YBR1127, YBR149W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/D-arabinose_1-dehydrogenase_(NAD(P)(+)) D-arabinose 1-dehydrogenase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.117 1.1.1.117] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ijr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ijr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ijr RCSB], [http://www.ebi.ac.uk/pdbsum/4ijr PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The primary role of yeast Ara1, previously mis-annotated as a D-arabinose dehydrogenase, is to catalyze the reduction of a variety of toxic alpha,beta-dicarbonyl compounds using NADPH as a cofactor at physiological pH levels. Here, crystal structures of Ara1 in apo and NADPH-complexed forms are presented at 2.10 and 2.00 A resolution, respectively. Ara1 exists as a homodimer, each subunit of which adopts an (alpha/beta)8-barrel structure and has a highly conserved cofactor-binding pocket. Structural comparison revealed that induced fit upon NADPH binding yielded an intact active-site pocket that recognizes the substrate. Moreover, the crystal structures combined with computational simulation defined an open substrate-binding site to accommodate various substrates that possess a dicarbonyl group.
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==Function==
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Structures of Saccharomyces cerevisiaeD-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition.,Hu XQ, Guo PC, Ma JD, Li WF Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1190-5. doi:, 10.1107/S1744309113026857. Epub 2013 Oct 26. PMID:24192347<ref>PMID:24192347</ref>
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[[http://www.uniprot.org/uniprot/ARA1_YEAST ARA1_YEAST]] Catalyzes the oxidation of D-arabinose, L-xylose, L-fucose and L-galactose in the presence of NADP(+).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4ijr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IJR OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:024192347</ref><references group="xtra"/><references/>
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__TOC__
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[[Category: Guo, P C.]]
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</StructureSection>
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[[Category: Hu, X Q.]]
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[[Category: Baker's yeast]]
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[[Category: Li, W F.]]
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[[Category: Guo, P C]]
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[[Category: Zhou, C Z.]]
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[[Category: Hu, X Q]]
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[[Category: Li, W F]]
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[[Category: Zhou, C Z]]
[[Category: Cytosol]]
[[Category: Cytosol]]
[[Category: Dehydrogenase]]
[[Category: Dehydrogenase]]

Revision as of 18:18, 21 December 2014

Crystal structure of Saccharomyces cerevisiae arabinose dehydrogenase Ara1 complexed with NADPH

4ijr, resolution 2.00Å

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