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4ik5

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{{STRUCTURE_4ik5| PDB=4ik5 | SCENE= }}
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==High resolution structure of Delta-REST-GCaMP3==
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===High resolution structure of Delta-REST-GCaMP3===
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<StructureSection load='4ik5' size='340' side='right' caption='[[4ik5]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23549615}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ik5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IK5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IK5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ik1|4ik1]], [[4ik3|4ik3]], [[4ik4|4ik4]], [[4ik8|4ik8]], [[4ik9|4ik9]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ik5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ik5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ik5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ik5 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Genetically encoded Ca(2+) indicators (GECI) are important for the measurement of Ca(2+) in vivo. GCaMP2, a widely-used GECI, has recently been iteratively improved. Among the improved variants, GCaMP3 exhibits significantly better fluorescent intensity. In this study, we developed a new GECI called GCaMPJ and determined the crystal structures of GCaMP3 and GCaMPJ. GCaMPJ has a 1.5-fold increase in fluorescence and 1.3-fold increase in calcium affinity over GCaMP3. Upon Ca(2+) binding, GCaMP3 exhibits both monomeric and dimeric forms. The structural superposition of these two forms reveals the role of Arg-376 in improving monomer performance. However, GCaMPJ seldom forms dimers under conditions similar to GCaMP3. St ructural and mutagenesis studies on Tyr-380 confirmed its importance in blocking the cpEGFP beta-barrel holes. Our study proposes an efficient tool for mapping Ca(2+) signals in intact organs to facilitate the further improvement of GCaMP sensors.
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==About this Structure==
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Structural insight into enhanced calcium indicator GCaMP3 and GCaMPJ to promote further improvement.,Chen Y, Song X, Ye S, Miao L, Zhu Y, Zhang RG, Ji G Protein Cell. 2013 Apr;4(4):299-309. doi: 10.1007/s13238-013-2103-4. Epub 2013, Apr 3. PMID:23549615<ref>PMID:23549615</ref>
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[[4ik5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IK5 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023549615</ref><references group="xtra"/><references/>
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</div>
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[[Category: Chen, Y.]]
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[[Category: Ji, G.]]
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==See Also==
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[[Category: Miao, L.]]
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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[[Category: Song, X.]]
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== References ==
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[[Category: Zhu, Y.]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Chen, Y]]
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[[Category: Ji, G]]
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[[Category: Miao, L]]
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[[Category: Song, X]]
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[[Category: Zhu, Y]]
[[Category: Beta-barrel]]
[[Category: Beta-barrel]]
[[Category: Calcium indicator]]
[[Category: Calcium indicator]]

Revision as of 18:19, 21 December 2014

High resolution structure of Delta-REST-GCaMP3

4ik5, resolution 2.50Å

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