4mna

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_4mna| PDB=4mna | SCENE= }}
+
==Crystal structure of the free FLS2 ectodomains==
-
===Crystal structure of the free FLS2 ectodomains===
+
<StructureSection load='4mna' size='340' side='right' caption='[[4mna]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_24114786}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4mna]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MNA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MNA FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mn8|4mn8]]</td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FLS2, At5g46330, MPL12.13, MPL12.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
 +
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mna OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mna RCSB], [http://www.ebi.ac.uk/pdbsum/4mna PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Flagellin perception in Arabidopsis is through recognition of its highly conserved N-terminal epitope (flg22) by flagellin-sensitive 2 (FLS2). Flg22 binding induces FLS2 heteromerization with BRASSINOSTEROID INSENSITIVE 1-associated kinase 1 (BAK1) and their reciprocal activation followed by plant immunity. Here, we report the crystal structure of FLS2 and BAK1 ectodomains complexed with flg22 at 3.06 angstroms. A conserved and a nonconserved site from the inner surface of the FLS2 solenoid recognize the C- and N-terminal segment of flg22, respectively, without oligomerization or conformational changes in the FLS2 ectodomain. Besides directly interacting with FLS2, BAK1 acts as a co-receptor by recognizing the C terminus of the FLS2-bound flg22. Our data reveal the molecular mechanisms underlying FLS2-BAK1 complex recognition of flg22 and provide insight into the immune receptor complex activation.
-
==Function==
+
Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex.,Sun Y, Li L, Macho AP, Han Z, Hu Z, Zipfel C, Zhou JM, Chai J Science. 2013 Nov 1;342(6158):624-8. doi: 10.1126/science.1243825. Epub 2013 Oct , 10. PMID:24114786<ref>PMID:24114786</ref>
-
[[http://www.uniprot.org/uniprot/FLS2_ARATH FLS2_ARATH]] Constitutes the pattern-recognition receptor (PPR) that determines the specific perception of flagellin (flg22), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Flagellin-binding to the receptor is the first step to initiate the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6), resulting in enhanced resistance against pathogens. Binding to the effector AvrPto1 from Pseudomonas syringae blocks the downstream plant immune response.<ref>PMID:10911994</ref> <ref>PMID:11340188</ref> <ref>PMID:11875555</ref> <ref>PMID:15085136</ref> <ref>PMID:19062288</ref> <ref>PMID:16377758</ref>
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[4mna]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MNA OCA].
+
</div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:024114786</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
 +
[[Category: Arath]]
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Non-specific serine/threonine protein kinase]]
-
[[Category: Chai, J.]]
+
[[Category: Chai, J]]
-
[[Category: Han, Z.]]
+
[[Category: Han, Z]]
-
[[Category: Sun, Y.]]
+
[[Category: Sun, Y]]
[[Category: Fls2]]
[[Category: Fls2]]
[[Category: Leucine-rich repeat]]
[[Category: Leucine-rich repeat]]
[[Category: Plant immunity]]
[[Category: Plant immunity]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 18:21, 21 December 2014

Crystal structure of the free FLS2 ectodomains

4mna, resolution 4.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4mna"
Personal tools