1sz1
From Proteopedia
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| - | [[Image:1sz1.gif|left|200px]] | + | [[Image:1sz1.gif|left|200px]] |
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| - | '''Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes''' | + | {{Structure |
| + | |PDB= 1sz1 |SIZE=350|CAPTION= <scene name='initialview01'>1sz1</scene>, resolution 6.21Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/tRNA_adenylyltransferase tRNA adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.25 2.7.7.25] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SZ1 is a [ | + | 1SZ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZ1 OCA]. |
==Reference== | ==Reference== | ||
| - | Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template., Xiong Y, Steitz TA, Nature. 2004 Aug 5;430(7000):640-5. PMID:[http:// | + | Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template., Xiong Y, Steitz TA, Nature. 2004 Aug 5;430(7000):640-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15295590 15295590] |
[[Category: Archaeoglobus fulgidus]] | [[Category: Archaeoglobus fulgidus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: trna]] | [[Category: trna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:11:30 2008'' |
Revision as of 12:11, 20 March 2008
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| , resolution 6.21Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | tRNA adenylyltransferase, with EC number 2.7.7.25 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes
Overview
Transfer RNA nucleotidyltransferases (CCA-adding enzymes) are responsible for the maturation or repair of the functional 3' end of tRNAs by means of the addition of the essential nucleotides CCA. However, it is unclear how tRNA nucleotidyltransferases polymerize CCA onto the 3' terminus of immature tRNAs without using a nucleic acid template. Here we describe the crystal structure of the Archaeoglobus fulgidus tRNA nucleotidyltransferase in complex with tRNA. We also present ternary complexes of this enzyme with both RNA duplex mimics of the tRNA acceptor stem that terminate with the nucleotides C74 or C75, as well as the appropriate incoming nucleoside 5'-triphosphates. A single nucleotide-binding pocket exists whose specificity for both CTP and ATP is determined by the protein side chain of Arg 224 and backbone phosphates of the tRNA, which are non-complementary to and thus exclude UTP and GTP. Discrimination between CTP or ATP at a given addition step and at termination arises from changes in the size and shape of the nucleotide binding site that is progressively altered by the elongating 3' end of the tRNA.
About this Structure
1SZ1 is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template., Xiong Y, Steitz TA, Nature. 2004 Aug 5;430(7000):640-5. PMID:15295590
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