1sza
From Proteopedia
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| - | [[Image:1sza.gif|left|200px]] | + | [[Image:1sza.gif|left|200px]] |
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| - | '''The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model''' | + | {{Structure |
| + | |PDB= 1sza |SIZE=350|CAPTION= <scene name='initialview01'>1sza</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= PCF11, YDR228C, YD9934.13C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1SZA is a [ | + | 1SZA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZA OCA]. |
==Reference== | ==Reference== | ||
| - | Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors., Meinhart A, Cramer P, Nature. 2004 Jul 8;430(6996):223-6. PMID:[http:// | + | Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors., Meinhart A, Cramer P, Nature. 2004 Jul 8;430(6996):223-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15241417 15241417] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cramer, P.]] | [[Category: Cramer, P.]] | ||
[[Category: Meinhart, A.]] | [[Category: Meinhart, A.]] | ||
| - | [[Category: arm | + | [[Category: arm repeat]] |
[[Category: pcf11]] | [[Category: pcf11]] | ||
[[Category: phosphoserine]] | [[Category: phosphoserine]] | ||
[[Category: rna polymerase ii ctd interacting domain]] | [[Category: rna polymerase ii ctd interacting domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:11:31 2008'' |
Revision as of 12:11, 20 March 2008
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| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | PCF11, YDR228C, YD9934.13C (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The RNA polymerase II CTD in mRNA processing: beta-turn recognition and beta-spiral model
Overview
During transcription, RNA polymerase (Pol) II synthesizes eukaryotic messenger RNA. Transcription is coupled to RNA processing by the carboxy-terminal domain (CTD) of Pol II, which consists of up to 52 repeats of the sequence Tyr 1-Ser 2-Pro 3-Thr 4-Ser 5-Pro 6-Ser 7 (refs 1, 2). After phosphorylation, the CTD binds tightly to a conserved CTD-interacting domain (CID) present in the proteins Pcf11 and Nrd1, which are essential and evolutionarily conserved factors for polyadenylation-dependent and -independent 3'-RNA processing, respectively. Here we describe the structure of a Ser 2-phosphorylated CTD peptide bound to the CID domain of Pcf11. The CTD motif Ser 2-Pro 3-Thr 4-Ser 5 forms a beta-turn that binds to a conserved groove in the CID domain. The Ser 2 phosphate group does not make direct contact with the CID domain, but may be recognized indirectly because it stabilizes the beta-turn with an additional hydrogen bond. Iteration of the peptide structure results in a compact beta-spiral model of the CTD. The model suggests that, during the mRNA transcription-processing cycle, compact spiral regions in the CTD are unravelled and regenerated in a phosphorylation-dependent manner.
About this Structure
1SZA is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors., Meinhart A, Cramer P, Nature. 2004 Jul 8;430(6996):223-6. PMID:15241417
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