3k1k

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{{STRUCTURE_3k1k| PDB=3k1k | SCENE= }}
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==Green fluorescent protein bound to minimizer nanobody==
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===Green fluorescent protein bound to minimizer nanobody===
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<StructureSection load='3k1k' size='340' side='right' caption='[[3k1k]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20010839}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3k1k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [http://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K1K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K1K FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3g9a|3g9a]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k1k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k1k RCSB], [http://www.ebi.ac.uk/pdbsum/3k1k PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k1/3k1k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). One nanobody could reversibly reduce GFP fluorescence by a factor of 5, whereas its displacement by a second nanobody caused an increase by a factor of 10. Structural analysis of GFP-nanobody complexes revealed that the two nanobodies induce subtle opposing changes in the chromophore environment, leading to altered absorption properties. Unlike conventional antibodies, the small, stable nanobodies are functional in living cells. Nanobody-induced changes were detected by ratio imaging and used to monitor protein expression and subcellular localization as well as translocation events such as the tamoxifen-induced nuclear localization of estrogen receptor. This work demonstrates that protein conformations can be manipulated and studied with nanobodies in living cells.
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==Function==
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Modulation of protein properties in living cells using nanobodies.,Kirchhofer A, Helma J, Schmidthals K, Frauer C, Cui S, Karcher A, Pellis M, Muyldermans S, Casas-Delucchi CS, Cardoso MC, Leonhardt H, Hopfner KP, Rothbauer U Nat Struct Mol Biol. 2010 Jan;17(1):133-8. Epub 2009 Dec 13. PMID:20010839<ref>PMID:20010839</ref>
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[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3k1k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [http://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K1K OCA].
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</div>
==See Also==
==See Also==
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*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
 
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*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
 
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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*[[Sandbox104|Sandbox104]]
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== References ==
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*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:020010839</ref><references group="xtra"/><references/>
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
[[Category: Vicugna pacos]]
[[Category: Vicugna pacos]]
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[[Category: Cardoso, M C.]]
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[[Category: Cardoso, M C]]
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[[Category: Cui, S.]]
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[[Category: Cui, S]]
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[[Category: Delucci, C C.]]
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[[Category: Delucci, C C]]
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[[Category: Frauer, C.]]
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[[Category: Frauer, C]]
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[[Category: Helma, J.]]
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[[Category: Helma, J]]
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[[Category: Hopfner, K P.]]
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[[Category: Hopfner, K P]]
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[[Category: Karcher, A.]]
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[[Category: Karcher, A]]
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[[Category: Kirchhofer, A.]]
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[[Category: Kirchhofer, A]]
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[[Category: Leonhardt, H.]]
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[[Category: Leonhardt, H]]
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[[Category: Muyldermans, S.]]
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[[Category: Muyldermans, S]]
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[[Category: Pellis, M.]]
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[[Category: Pellis, M]]
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[[Category: Rothbauer, U.]]
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[[Category: Rothbauer, U]]
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[[Category: Schmidthals, K.]]
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[[Category: Schmidthals, K]]
[[Category: Antibody-complex]]
[[Category: Antibody-complex]]
[[Category: Chromophore]]
[[Category: Chromophore]]

Revision as of 18:31, 21 December 2014

Green fluorescent protein bound to minimizer nanobody

3k1k, resolution 2.15Å

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