1t00

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[[Image:1t00.gif|left|200px]]<br /><applet load="1t00" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1t00.gif|left|200px]]
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caption="1t00, resolution 1.51&Aring;" />
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'''The structure of thioredoxin from S. coelicolor'''<br />
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{{Structure
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|PDB= 1t00 |SIZE=350|CAPTION= <scene name='initialview01'>1t00</scene>, resolution 1.51&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= TRXA, SCO3889, SCH24.11C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])
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}}
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'''The structure of thioredoxin from S. coelicolor'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1T00 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T00 OCA].
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1T00 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T00 OCA].
==Reference==
==Reference==
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Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor., Stefankova P, Maderova J, Barak I, Kollarova M, Otwinowski Z, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt, 2):164-8. Epub 2005 Jan 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16510983 16510983]
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Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor., Stefankova P, Maderova J, Barak I, Kollarova M, Otwinowski Z, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt, 2):164-8. Epub 2005 Jan 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16510983 16510983]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces coelicolor]]
[[Category: Streptomyces coelicolor]]
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[[Category: thioredoxin]]
[[Category: thioredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:11:49 2008''

Revision as of 12:11, 20 March 2008


PDB ID 1t00

Drag the structure with the mouse to rotate
, resolution 1.51Å
Gene: TRXA, SCO3889, SCH24.11C (Streptomyces coelicolor)
Coordinates: save as pdb, mmCIF, xml



The structure of thioredoxin from S. coelicolor


Overview

Thioredoxins are ubiquitous proteins that serve as reducing agents and general protein disulfide reductases. In turn, they are reduced by electrons obtained from the NADPH-containing thioredoxin reductase. Thioredoxins have been isolated and characterized from a large number of organisms. The Gram-positive bacterium Streptomyces coelicolor contains three thioredoxins that are involved in unknown biological processes. trxA from S. coelicolor was cloned and expressed in Escherichia coli and the protein purified and crystallized using the hanging-drop method of vapour diffusion. The crystal structure of thioredoxin A has been determined at 1.5 A resolution using a synchrotron-radiation source. The protein reveals a thioredoxin-like fold with a typical CXXC active site. The crystal exhibits the symmetry of space group P2(1)2(1)2, with unit-cell parameters a = 43.6, b = 71.8, c = 33.2 A.

About this Structure

1T00 is a Single protein structure of sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA.

Reference

Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor., Stefankova P, Maderova J, Barak I, Kollarova M, Otwinowski Z, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Feb 1;61(Pt, 2):164-8. Epub 2005 Jan 8. PMID:16510983

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