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4k6m

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{{STRUCTURE_4k6m| PDB=4k6m | SCENE= }}
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==Crystal Structure of the full-length Japanese encephalitis virus NS5==
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===Crystal Structure of the full-length Japanese encephalitis virus NS5===
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<StructureSection load='4k6m' size='340' side='right' caption='[[4k6m]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23950717}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4k6m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_encephalitis Japanese encephalitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K6M FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NS5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11072 Japanese encephalitis])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k6m RCSB], [http://www.ebi.ac.uk/pdbsum/4k6m PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The flavivirus NS5 harbors a methyltransferase (MTase) in its N-terminal approximately 265 residues and an RNA-dependent RNA polymerase (RdRP) within the C-terminal part. One of the major interests and challenges in NS5 is to understand the interplay between RdRP and MTase as a unique natural fusion protein in viral genome replication and cap formation. Here, we report the first crystal structure of the full-length flavivirus NS5 from Japanese encephalitis virus. The structure completes the vision for polymerase motifs F and G, and depicts defined intra-molecular interactions between RdRP and MTase. Key hydrophobic residues in the RdRP-MTase interface are highly conserved in flaviviruses, indicating the biological relevance of the observed conformation. Our work paves the way for further dissection of the inter-regulations of the essential enzymatic activities of NS5 and exploration of possible other conformations of NS5 under different circumstances.
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==Function==
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Crystal Structure of the full-length Japanese encephalitis virus NS5 reveals a conserved methyltransferase-polymerase interface.,Lu G, Gong P PLoS Pathog. 2013;9(8):e1003549. doi: 10.1371/journal.ppat.1003549. Epub 2013 Aug, 8. PMID:23950717<ref>PMID:23950717</ref>
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[[http://www.uniprot.org/uniprot/Q5NT71_9FLAV Q5NT71_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4k6m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_encephalitis Japanese encephalitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K6M OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023950717</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Japanese encephalitis]]
[[Category: Japanese encephalitis]]
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[[Category: Gong, P.]]
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[[Category: Gong, P]]
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[[Category: Lu, G.]]
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[[Category: Lu, G]]
[[Category: Methyltransferase]]
[[Category: Methyltransferase]]
[[Category: Rna-dependent rna polymerase]]
[[Category: Rna-dependent rna polymerase]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 18:38, 21 December 2014

Crystal Structure of the full-length Japanese encephalitis virus NS5

4k6m, resolution 2.60Å

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