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1t0k
From Proteopedia
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| - | [[Image:1t0k.gif|left|200px]] | + | [[Image:1t0k.gif|left|200px]] |
| - | + | ||
| - | '''Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex''' | + | {{Structure |
| + | |PDB= 1t0k |SIZE=350|CAPTION= <scene name='initialview01'>1t0k</scene>, resolution 3.24Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MTT:MALTOTETRAOSE'>MTT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= MALE, B4034, Z5632, ECS5017 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), RPL30, RPL32, YGL030W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1T0K is a [ | + | 1T0K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entries 1CK5, 1CK8, 1CN8 and 1CN9. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0K OCA]. |
==Reference== | ==Reference== | ||
| - | Joint X-ray and NMR refinement of the yeast L30e-mRNA complex., Chao JA, Williamson JR, Structure. 2004 Jul;12(7):1165-76. PMID:[http:// | + | Joint X-ray and NMR refinement of the yeast L30e-mRNA complex., Chao JA, Williamson JR, Structure. 2004 Jul;12(7):1165-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15242593 15242593] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: ribosomal protein l30e]] | [[Category: ribosomal protein l30e]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:12:04 2008'' |
Revision as of 12:12, 20 March 2008
| |||||||
| , resolution 3.24Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | MALE, B4034, Z5632, ECS5017 (Escherichia coli), RPL30, RPL32, YGL030W (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Joint X-ray and NMR Refinement of Yeast L30e-mRNA complex
Overview
L30e, a Saccharomyces cervisiae ribosomal protein, regulates its own expression by binding to a purine-rich asymmetric internal loop located in both its pre-mRNA and mature mRNA. A crystal structure of an MBP-L30e fusion protein in complex with an RNA containing the pre-mRNA regulatory site was solved at 3.24 A. Interestingly, the structure of the RNA differed from that observed in a previously determined NMR structure of the complex. Analysis of the NMR data led to the identification of a single imino proton resonance in the internal loop that had been incorrectly assigned and was principally responsible for the erroneous RNA structure. A structure refinement was performed using both the X-ray diffraction data and the NMR-derived distance and angle restraints. The joint NMR and X-ray refinement resulted in improved stereochemistry and lower crystallographic R factors. The RNA internal loop of the MBP-L30e-mRNA complex adopts the canonical K-turn fold.
About this Structure
1T0K is a Protein complex structure of sequences from Escherichia coli and Saccharomyces cerevisiae. This structure supersedes the now removed PDB entries 1CK5, 1CK8, 1CN8 and 1CN9. Full crystallographic information is available from OCA.
Reference
Joint X-ray and NMR refinement of the yeast L30e-mRNA complex., Chao JA, Williamson JR, Structure. 2004 Jul;12(7):1165-76. PMID:15242593
Page seeded by OCA on Thu Mar 20 14:12:04 2008
