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4o8a

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{{STRUCTURE_4o8a| PDB=4o8a | SCENE= }}
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==First structure of a proline utilization A proline dehydrogenase domain==
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===First structure of a proline utilization A proline dehydrogenase domain===
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<StructureSection load='4o8a' size='340' side='right' caption='[[4o8a]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_12514740}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4o8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1k87 1k87]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O8A FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2OP:(2S)-2-HYDROXYPROPANOIC+ACID'>2OP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1014, JW0999, poaA, putA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o8a RCSB], [http://www.ebi.ac.uk/pdbsum/4o8a PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The PutA flavoprotein from Escherichia coli plays multiple roles in proline catabolism by functioning as a membrane-associated bi-functional enzyme and a transcriptional repressor of proline utilization genes. The human homolog of the PutA proline dehydrogenase (PRODH) domain is critical in p53-mediated apoptosis and schizophrenia. Here we report the crystal structure of a 669-residue truncated form of PutA that shows both PRODH and DNA-binding activities, representing the first structure of a PutA protein and a PRODH enzyme from any organism. The structure is a domain-swapped dimer with each subunit comprising three domains: a helical dimerization arm, a 120-residue domain containing a three-helix bundle similar to that in the helix-turn-helix superfamily of DNA-binding proteins and a beta/alpha-barrel PRODH domain with a bound lactate inhibitor. Analysis of the structure provides insight into the mechanism of proline oxidation to pyrroline-5-carboxylate, and functional studies of a mutant protein suggest that the DNA-binding domain is located within the N-terminal 261 residues of E. coli PutA.
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==Function==
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Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein.,Lee YH, Nadaraia S, Gu D, Becker DF, Tanner JJ Nat Struct Biol. 2003 Feb;10(2):109-14. PMID:12514740<ref>PMID:12514740</ref>
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[[http://www.uniprot.org/uniprot/PUTA_ECOLI PUTA_ECOLI]] Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4o8a]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1k87 1k87]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O8A OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:012514740</ref><references group="xtra"/><references/>
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__TOC__
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[[Category: Tanner, J J.]]
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</StructureSection>
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[[Category: Ecoli]]
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[[Category: Tanner, J J]]
[[Category: Aldehyde dehydrogenase]]
[[Category: Aldehyde dehydrogenase]]
[[Category: Flavoenzyme]]
[[Category: Flavoenzyme]]

Revision as of 18:43, 21 December 2014

First structure of a proline utilization A proline dehydrogenase domain

4o8a, resolution 2.00Å

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