4n7k

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{{STRUCTURE_4n7k| PDB=4n7k | SCENE= }}
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==Zinc Substituted Reaction Center of the Rhodobacter sphaeroides==
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===Zinc Substituted Reaction Center of the Rhodobacter sphaeroides===
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<StructureSection load='4n7k' size='340' side='right' caption='[[4n7k]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24316146}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4n7k]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N7K FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2GO:[METHYL+9-ACETYL-14-ETHYL-20-HYDROXY-4,8,13,18-TETRAMETHYL-3-{3-OXO-3-[(3,7,11,15-TETRAMETHYLHEXADEC-2-EN-1-YL)OXY]PROPYL}-3,4,20,21-TETRADEHYDROPHORBINE-21-CARBOXYLATATO(2-)-KAPPA~4~N~23~,N~24~,N~25~,N~26~]ZINC'>2GO</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GGD:NONADEC-10-ENOIC+ACID+2-[3,4-DIHYDROXY-6-HYDROXYMETHYL-5-(3,4,5-TRIHYDROXY-6-HYDROXYMETHYL-TETRAHYDRO-PYRAN-2-YLOXY)-TETRAHYDRO-PYRAN-2-YLOXY]-1-OCTADEC-9-ENOYLOXYMETHYL-ETHYL+ESTER'>GGD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PC1:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PC1</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPO:SPHEROIDENE'>SPO</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j8c|2j8c]], [[4n7l|4n7l]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pufL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 "Rhodococcus capsulatus" Molisch 1907]), pufM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 "Rhodococcus capsulatus" Molisch 1907])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n7k RCSB], [http://www.ebi.ac.uk/pdbsum/4n7k PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Zn-BChl-containing reaction center (RC) produced in a bchD (magnesium chelatase) mutant of Rhodobacter sphaeroides assembles with six Zn-bacteriochlorophylls (Zn-BChls) in place of four Mg-containing bacteriochlorophylls (BChls) and two bacteriopheophytins (BPhes). This protein presents unique opportunities for studying biological electron transfer, as Zn-containing chlorins can exist in 4-, 5-, and (theoretically) 6-coordinate states within the RC. In this paper, the electron transfer perturbations attributed exclusively to coordination state effects are separated from those attributed to the presence, absence, or type of metal in the bacteriochlorin at the HA pocket of the RC. The presence of a 4-coordinate Zn2+ ion in the HA bacteriochlorin instead of BPhe results in a small decrease in the rates of the P*--&gt;P+HA---&gt;P+QA- electron transfer, and the charge separation yield is not greatly perturbed; however coordination of the Zn2+ by a fifth ligand provided by a histidine residue results in a larger rate decrease and yield loss. We also report the first crystal structure of a Zn-BChl-containing RC, confirming that the HA Zn-BChl was either 4- or 5-coordinate in the two types of Zn-BChl-containing RCs studied here. Interestingly, a large degree of disorder, in combination with a relatively weak anomalous difference electron density was found in the HB pocket. These data, in combination with spectroscopic results, indicate partial occupancy of this binding pocket. These findings provide insights into the use of BPhe as the bacteriochlorin pigment of choice at HA in both BChl- and Zn-BChl-containing RCs found in nature.
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==Function==
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Structural and kinetic properties of Rhodobacter sphaeroides photosynthetic reaction centers containing exclusively Zn-coordinated bacteriochlorophyll as bacteriochlorin cofactors.,Saer RG, Pan J, Hardjasa A, Lin S, Rosell F, Mauk AG, Woodbury NW, Murphy ME, Beatty JT Biochim Biophys Acta. 2013 Dec 5;1837(3):366-374. doi:, 10.1016/j.bbabio.2013.11.015. PMID:24316146<ref>PMID:24316146</ref>
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[[http://www.uniprot.org/uniprot/RCEH_RHOSH RCEH_RHOSH]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/RCEM_RHOSH RCEM_RHOSH]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/RCEL_RHOSH RCEL_RHOSH]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4n7k]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7K OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:024316146</ref><references group="xtra"/><references/>
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__TOC__
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[[Category: Hardjasa, A.]]
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</StructureSection>
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[[Category: Murphy, M E.P.]]
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[[Category: Rhodococcus capsulatus molisch 1907]]
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[[Category: Hardjasa, A]]
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[[Category: Murphy, M E.P]]
[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
[[Category: Zinc bacteriochlorophyll]]
[[Category: Zinc bacteriochlorophyll]]

Revision as of 18:50, 21 December 2014

Zinc Substituted Reaction Center of the Rhodobacter sphaeroides

4n7k, resolution 2.85Å

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