1t2t
From Proteopedia
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| - | [[Image:1t2t.gif|left|200px]] | + | [[Image:1t2t.gif|left|200px]] |
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| - | '''Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site''' | + | {{Structure |
| + | |PDB= 1t2t |SIZE=350|CAPTION= <scene name='initialview01'>1t2t</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= ITEVIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T4]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T2T is a [ | + | 1T2T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2T OCA]. |
==Reference== | ==Reference== | ||
| - | Intron-encoded homing endonuclease I-TevI also functions as a transcriptional autorepressor., Edgell DR, Derbyshire V, Van Roey P, LaBonne S, Stanger MJ, Li Z, Boyd TM, Shub DA, Belfort M, Nat Struct Mol Biol. 2004 Oct;11(10):936-44. Epub 2004 Sep 7. PMID:[http:// | + | Intron-encoded homing endonuclease I-TevI also functions as a transcriptional autorepressor., Edgell DR, Derbyshire V, Van Roey P, LaBonne S, Stanger MJ, Li Z, Boyd TM, Shub DA, Belfort M, Nat Struct Mol Biol. 2004 Oct;11(10):936-44. Epub 2004 Sep 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15361856 15361856] |
[[Category: Bacteriophage t4]] | [[Category: Bacteriophage t4]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:13:00 2008'' |
Revision as of 12:13, 20 March 2008
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| , resolution 2.50Å | |||||||
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| Ligands: | |||||||
| Gene: | ITEVIR (Bacteriophage T4) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site
Overview
Customary binding sites of intron-encoded homing endonucleases lie within cognate intronless alleles, at the so-called homing sites. Here, we describe a novel, high-affinity binding site for I-TevI endonuclease, encoded within the group I td intron of phage T4. This site is an operator that overlaps the T4 late promoter, which drives I-TevI expression from within the td intron. I-TevI binds the operator and homing sites with equal affinity, and functions as a transcriptional autorepressor. Distinct sequence and spacing requirements of the catalytic domain result in reduced cleavage activity on operator DNA. Crystallographic studies showed that the overall interactions of the DNA-binding domain with the operator and homing sites are similar, but have some different hydrogen-bonding contacts. We present a model in which the flexibility in protein-DNA interactions allows I-TevI to bind variant intronless alleles to promote intron mobility while facilitating its function in autorepression, and thereby persistence in its host.
About this Structure
1T2T is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.
Reference
Intron-encoded homing endonuclease I-TevI also functions as a transcriptional autorepressor., Edgell DR, Derbyshire V, Van Roey P, LaBonne S, Stanger MJ, Li Z, Boyd TM, Shub DA, Belfort M, Nat Struct Mol Biol. 2004 Oct;11(10):936-44. Epub 2004 Sep 7. PMID:15361856
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