1t3n

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1t3n.gif|left|200px]]<br /><applet load="1t3n" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1t3n.gif|left|200px]]
-
caption="1t3n, resolution 2.3&Aring;" />
+
 
-
'''Structure of the catalytic core of DNA polymerase Iota in complex with DNA and dTTP'''<br />
+
{{Structure
 +
|PDB= 1t3n |SIZE=350|CAPTION= <scene name='initialview01'>1t3n</scene>, resolution 2.3&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=TTP:THYMIDINE-5'-TRIPHOSPHATE'>TTP</scene>
 +
|ACTIVITY=
 +
|GENE= POLI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
 +
}}
 +
 
 +
'''Structure of the catalytic core of DNA polymerase Iota in complex with DNA and dTTP'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1T3N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=TTP:'>TTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3N OCA].
+
1T3N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3N OCA].
==Reference==
==Reference==
-
Replication by human DNA polymerase-iota occurs by Hoogsteen base-pairing., Nair DT, Johnson RE, Prakash S, Prakash L, Aggarwal AK, Nature. 2004 Jul 15;430(6997):377-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15254543 15254543]
+
Replication by human DNA polymerase-iota occurs by Hoogsteen base-pairing., Nair DT, Johnson RE, Prakash S, Prakash L, Aggarwal AK, Nature. 2004 Jul 15;430(6997):377-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15254543 15254543]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 24: Line 33:
[[Category: protein]]
[[Category: protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:09:35 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:13:22 2008''

Revision as of 12:13, 20 March 2008

Image:1t3n.gif


PDB ID 1t3n

Drag the structure with the mouse to rotate
, resolution 2.3Å
Ligands: and
Gene: POLI (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Structure of the catalytic core of DNA polymerase Iota in complex with DNA and dTTP


Overview

Almost all DNA polymerases show a strong preference for incorporating the nucleotide that forms the correct Watson-Crick base pair with the template base. In addition, the catalytic efficiencies with which any given polymerase forms the four possible correct base pairs are roughly the same. Human DNA polymerase-iota (hPoliota), a member of the Y family of DNA polymerases, is an exception to these rules. hPoliota incorporates the correct nucleotide opposite a template adenine with a several hundred to several thousand fold greater efficiency than it incorporates the correct nucleotide opposite a template thymine, whereas its efficiency for correct nucleotide incorporation opposite a template guanine or cytosine is intermediate between these two extremes. Here we present the crystal structure of hPoliota bound to a template primer and an incoming nucleotide. The structure reveals a polymerase that is 'specialized' for Hoogsteen base-pairing, whereby the templating base is driven to the syn conformation. Hoogsteen base-pairing offers a basis for the varied efficiencies and fidelities of hPoliota opposite different template bases, and it provides an elegant mechanism for promoting replication through minor-groove purine adducts that interfere with replication.

About this Structure

1T3N is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Replication by human DNA polymerase-iota occurs by Hoogsteen base-pairing., Nair DT, Johnson RE, Prakash S, Prakash L, Aggarwal AK, Nature. 2004 Jul 15;430(6997):377-80. PMID:15254543

Page seeded by OCA on Thu Mar 20 14:13:22 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t3n"
Personal tools