1t4y
From Proteopedia
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| - | [[Image:1t4y.jpg|left|200px]] | + | [[Image:1t4y.jpg|left|200px]] |
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| - | '''Solution structure of the N-terminal domain of Synechococcus elongatus SasA (average minimized structure)''' | + | {{Structure |
| + | |PDB= 1t4y |SIZE=350|CAPTION= <scene name='initialview01'>1t4y</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SASA, SARS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32046 Synechococcus elongatus]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of the N-terminal domain of Synechococcus elongatus SasA (average minimized structure)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T4Y is a [ | + | 1T4Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4Y OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the N-terminal domain of the circadian clock-associated histidine kinase SasA., Vakonakis I, Klewer DA, Williams SB, Golden SS, LiWang AC, J Mol Biol. 2004 Sep 3;342(1):9-17. PMID:[http:// | + | Structure of the N-terminal domain of the circadian clock-associated histidine kinase SasA., Vakonakis I, Klewer DA, Williams SB, Golden SS, LiWang AC, J Mol Biol. 2004 Sep 3;342(1):9-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313603 15313603] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Synechococcus elongatus]] | [[Category: Synechococcus elongatus]] | ||
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[[Category: thioredoxin fold]] | [[Category: thioredoxin fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:13:52 2008'' |
Revision as of 12:13, 20 March 2008
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| Gene: | SASA, SARS (Synechococcus elongatus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the N-terminal domain of Synechococcus elongatus SasA (average minimized structure)
Overview
Circadian oscillators are endogenous biological systems that generate the approximately 24 hour temporal pattern of biological processes and confer a reproductive fitness advantage to their hosts. The cyanobacterial clock is the simplest known and the only clock system for which structural information for core component proteins, in this case KaiA, KaiB and KaiC, is available. SasA, a clock-associated histidine kinase, is necessary for robustness of the circadian rhythm of gene expression and implicated in clock output. The N-terminal domain of SasA (N-SasA) interacts directly with KaiC and likely functions as the sensory domain controlling the SasA histidine kinase activity. N-SasA and KaiB share significant sequence similarity and, thus, it has been proposed that they would be structurally similar and may even compete for KaiC binding. Here, we report the NMR structure of N-SasA and show it to be different from that of KaiB. The structural comparisons provide no clear details to suggest competition of SasA and KaiB for KaiC binding. N-SasA adopts a canonical thioredoxin fold but lacks the catalytic cysteine residues. A patch of conserved, solvent-exposed residues is found near the canonical thioredoxin active site. We suggest that this surface is used by N-SasA for protein-protein interactions. Our analysis suggests that the structural differences between N-SasA and KaiB are the result of only a few critical amino acid substitutions.
About this Structure
1T4Y is a Single protein structure of sequence from Synechococcus elongatus. Full crystallographic information is available from OCA.
Reference
Structure of the N-terminal domain of the circadian clock-associated histidine kinase SasA., Vakonakis I, Klewer DA, Williams SB, Golden SS, LiWang AC, J Mol Biol. 2004 Sep 3;342(1):9-17. PMID:15313603
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