1t6o
From Proteopedia
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| - | [[Image:1t6o.gif|left|200px]] | + | [[Image:1t6o.gif|left|200px]] |
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| - | '''Nucleocapsid-binding domain of the measles virus P protein (amino acids 457-507) in complex with amino acids 486-505 of the measles virus N protein''' | + | {{Structure |
| + | |PDB= 1t6o |SIZE=350|CAPTION= <scene name='initialview01'>1t6o</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= P/V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11234 Measles virus]), N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11234 Measles virus]) | ||
| + | }} | ||
| + | |||
| + | '''Nucleocapsid-binding domain of the measles virus P protein (amino acids 457-507) in complex with amino acids 486-505 of the measles virus N protein''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1T6O is a [ | + | 1T6O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Measles_virus Measles virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6O OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the attachment of a paramyxoviral polymerase to its template., Kingston RL, Hamel DJ, Gay LS, Dahlquist FW, Matthews BW, Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8301-6. Epub 2004 May 24. PMID:[http:// | + | Structural basis for the attachment of a paramyxoviral polymerase to its template., Kingston RL, Hamel DJ, Gay LS, Dahlquist FW, Matthews BW, Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8301-6. Epub 2004 May 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15159535 15159535] |
[[Category: Measles virus]] | [[Category: Measles virus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: four helix bundle]] | [[Category: four helix bundle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:14:31 2008'' |
Revision as of 12:14, 20 March 2008
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| , resolution 2.0Å | |||||||
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| Gene: | P/V (Measles virus), N (Measles virus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Nucleocapsid-binding domain of the measles virus P protein (amino acids 457-507) in complex with amino acids 486-505 of the measles virus N protein
Overview
The nucleocapsid of measles virus is the template for viral RNA synthesis and is generated through packaging of the genomic RNA by the nucleocapsid protein (N). The viral polymerase associates with the nucleocapsid through a small, trihelical binding domain at the carboxyl terminus of the phosphoprotein (P). Translocation of the polymerase along the nucleocapsid during RNA synthesis is thought to involve the repeated attachment and release of the binding domain. We have investigated the interaction between the binding domain from measles P (amino acids 457-507) and the sequence it recognizes within measles N (amino acids 477-505). By using both solution NMR spectroscopy and x-ray crystallography, we show that N(487-503) binds as a helix to the surface created by the second (alpha2) and third (alpha3) helices of P(457-507), in an orientation parallel to the helix alpha3, creating a four-helix bundle. The binding interface is tightly packed and dominated by hydrophobic amino acids. Binding and folding of N(487-503) are coupled. However, when not bound to P, N(487-503) does not resemble a statistical random coil but instead exists in a loosely structured state that mimics the bound conformation. We propose that before diffusional encounter, the ensemble of accessible conformations for N(487-503) is biased toward structures capable of binding P, facilitating rapid association of the two proteins. This study provides a structural analysis of polymerase-template interactions in a paramyxovirus and presents an example of a protein-protein interaction that must be only transiently maintained as part of its normal function.
About this Structure
1T6O is a Protein complex structure of sequences from Measles virus. Full crystallographic information is available from OCA.
Reference
Structural basis for the attachment of a paramyxoviral polymerase to its template., Kingston RL, Hamel DJ, Gay LS, Dahlquist FW, Matthews BW, Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8301-6. Epub 2004 May 24. PMID:15159535
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