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1t8z

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[[Image:1t8z.jpg|left|200px]]<br /><applet load="1t8z" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1t8z.jpg|left|200px]]
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caption="1t8z, resolution 1.45&Aring;" />
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'''Atomic Structure of A Novel Tryptophan-Zipper Pentamer'''<br />
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{{Structure
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|PDB= 1t8z |SIZE=350|CAPTION= <scene name='initialview01'>1t8z</scene>, resolution 1.45&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=12P:DODECAETHYLENE GLYCOL'>12P</scene>
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|ACTIVITY=
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|GENE= LPP, MLPA, MULI, B1677, C2072, Z2705, ECS2384, SF1706, S1839 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''Atomic Structure of A Novel Tryptophan-Zipper Pentamer'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1T8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=12P:'>12P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8Z OCA].
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1T8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8Z OCA].
==Reference==
==Reference==
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Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15520380 15520380]
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Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15520380 15520380]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: tryptophan-zipper]]
[[Category: tryptophan-zipper]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:11:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:15:25 2008''

Revision as of 12:15, 20 March 2008

Image:1t8z.jpg


PDB ID 1t8z

Drag the structure with the mouse to rotate
, resolution 1.45Å
Ligands: and
Gene: LPP, MLPA, MULI, B1677, C2072, Z2705, ECS2384, SF1706, S1839 (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



Atomic Structure of A Novel Tryptophan-Zipper Pentamer


Overview

Coiled-coil motifs are ubiquitous mediators of specific protein-protein interactions through the formation of interlocking hydrophobic seams between alpha-helical chains. Residues that form these seams occur at the first (a) and fourth (d) positions of a characteristic 7-aa repeat and are primarily aliphatic. The potential of aromatic residues to promote helix association in a coiled coil was explored by engineering a "Trp-zipper" protein with Trp residues at all 14 a and d positions. The protein forms a discrete, stable, alpha-helical pentamer in water at physiological pH. Its 1.45-A crystal structure reveals a parallel, five-stranded coiled coil, a previously uncharacterized type of "knobs-into-holes" packing interaction between interfacial Trp side chains, and an unusual approximately 8-A-diameter axial channel lined with indole rings that is filled with polyethylene glycol 400 and water and sulfate ion molecules. The engineered Trp-zipper pentamer enlarges current views of coiled-coil assembly, molecular recognition, and protein engineering, and may serve as a soluble model for membrane ion channels.

About this Structure

1T8Z is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:15520380

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