1ca5
From Proteopedia
(Difference between revisions)
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| - | + | ==INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA== | |
| - | + | <StructureSection load='1ca5' size='340' side='right' caption='[[1ca5]], [[Resolution|resolution]] 2.20Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1ca5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CA5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ca5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ca5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ca5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ca5 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation. | ||
| - | + | Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs.,Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116<ref>PMID:11031116</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Sulfolobus acidocaldarius]] | [[Category: Sulfolobus acidocaldarius]] | ||
| - | [[Category: Gao, Y G | + | [[Category: Gao, Y G]] |
| - | [[Category: Robinson, H | + | [[Category: Robinson, H]] |
| - | [[Category: Shriver, J W | + | [[Category: Shriver, J W]] |
| - | [[Category: Su, S | + | [[Category: Su, S]] |
| - | [[Category: Wang, A H.J | + | [[Category: Wang, A H.J]] |
[[Category: Chromosomal protein]] | [[Category: Chromosomal protein]] | ||
[[Category: Dna binding]] | [[Category: Dna binding]] | ||
Revision as of 05:37, 22 December 2014
INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA
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