1tc3
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1tc3.gif|left|200px]] | + | [[Image:1tc3.gif|left|200px]] |
| - | + | ||
| - | '''TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS''' | + | {{Structure |
| + | |PDB= 1tc3 |SIZE=350|CAPTION= <scene name='initialview01'>1tc3</scene>, resolution 2.450Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= TC3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]) | ||
| + | }} | ||
| + | |||
| + | '''TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1TC3 is a [ | + | 1TC3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TC3 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the specific DNA-binding domain of Tc3 transposase of C.elegans in complex with transposon DNA., van Pouderoyen G, Ketting RF, Perrakis A, Plasterk RH, Sixma TK, EMBO J. 1997 Oct 1;16(19):6044-54. PMID:[http:// | + | Crystal structure of the specific DNA-binding domain of Tc3 transposase of C.elegans in complex with transposon DNA., van Pouderoyen G, Ketting RF, Perrakis A, Plasterk RH, Sixma TK, EMBO J. 1997 Oct 1;16(19):6044-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9312061 9312061] |
[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: transposase]] | [[Category: transposase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:16:28 2008'' |
Revision as of 12:16, 20 March 2008
| |||||||
| , resolution 2.450Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | TC3A (Caenorhabditis elegans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS
Overview
The crystal structure of the complex between the N-terminal DNA-binding domain of Tc3 transposase and an oligomer of transposon DNA has been determined. The specific DNA-binding domain contains three alpha-helices, of which two form a helix-turn-helix (HTH) motif. The recognition of transposon DNA by the transposase is mediated through base-specific contacts and complementarity between protein and sequence-dependent deformations of the DNA. The HTH motif makes four base-specific contacts with the major groove, and the N-terminus makes three base-specific contacts with the minor groove. The DNA oligomer adopts a non-linear B-DNA conformation, made possible by a stretch of seven G:C base pairs at one end and a TATA sequence towards the other end. Extensive contacts (seven salt bridges and 16 hydrogen bonds) of the protein with the DNA backbone allow the protein to probe and recognize the sequence-dependent DNA deformation. The DNA-binding domain forms a dimer in the crystals. Each monomer binds a separate transposon end, implying that the dimer plays a role in synapsis, necessary for the simultaneous cleavage of both transposon termini.
About this Structure
1TC3 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Crystal structure of the specific DNA-binding domain of Tc3 transposase of C.elegans in complex with transposon DNA., van Pouderoyen G, Ketting RF, Perrakis A, Plasterk RH, Sixma TK, EMBO J. 1997 Oct 1;16(19):6044-54. PMID:9312061
Page seeded by OCA on Thu Mar 20 14:16:28 2008
