1te6

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[[Image:1te6.gif|left|200px]]<br /><applet load="1te6" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1te6.gif|left|200px]]
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caption="1te6, resolution 1.80&Aring;" />
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'''Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom'''<br />
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{{Structure
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|PDB= 1te6 |SIZE=350|CAPTION= <scene name='initialview01'>1te6</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11]
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|GENE= ENO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1TE6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TE6 OCA].
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1TE6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TE6 OCA].
==Reference==
==Reference==
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Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase., Chai G, Brewer JM, Lovelace LL, Aoki T, Minor W, Lebioda L, J Mol Biol. 2004 Aug 20;341(4):1015-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15289101 15289101]
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Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase., Chai G, Brewer JM, Lovelace LL, Aoki T, Minor W, Lebioda L, J Mol Biol. 2004 Aug 20;341(4):1015-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15289101 15289101]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Phosphopyruvate hydratase]]
[[Category: Phosphopyruvate hydratase]]
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[[Category: TRS]]
[[Category: TRS]]
[[Category: enolase]]
[[Category: enolase]]
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[[Category: isozymes]]
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[[Category: isozyme]]
[[Category: negative cooperativity]]
[[Category: negative cooperativity]]
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[[Category: neurons]]
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[[Category: neuron]]
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[[Category: surface charges]]
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[[Category: surface charge]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:12:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:17:13 2008''

Revision as of 12:17, 20 March 2008

Image:1te6.gif


PDB ID 1te6

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands: , , and
Gene: ENO2 (Homo sapiens)
Activity: Phosphopyruvate hydratase, with EC number 4.2.1.11
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom


Overview

Human neuron-specific enolase (NSE) or isozyme gamma has been expressed with a C-terminal His-tag in Escherichia coli. The enzyme has been purified, crystallized and its crystal structure determined. In the crystals the enzyme forms the asymmetric complex NSE x Mg2 x SO4/NSE x Mg x Cl, where "/" separates the dimer subunits. The subunit that contains the sulfate (or phosphate) ion and two magnesium ions is in the closed conformation observed in enolase complexes with the substrate or its analogues; the other subunit is in the open conformation observed in enolase subunits without bound substrate or analogues. This indicates negative cooperativity for ligand binding between subunits. Electrostatic charge differences between isozymes alpha and gamma, -19 at physiological pH, are concentrated in the regions of the molecular surface that are negatively charged in alpha, i.e. surface areas negatively charged in alpha are more negatively charged in gamma, while areas that are neutral or positively charged tend to be charge-conserved.

About this Structure

1TE6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase., Chai G, Brewer JM, Lovelace LL, Aoki T, Minor W, Lebioda L, J Mol Biol. 2004 Aug 20;341(4):1015-21. PMID:15289101

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