1ted
From Proteopedia
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| - | [[Image:1ted.jpg|left|200px]] | + | [[Image:1ted.jpg|left|200px]] |
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| - | '''Crystal structure of a type III polyketide synthase PKS18 from Mycobacterium tuberculosis''' | + | {{Structure |
| + | |PDB= 1ted |SIZE=350|CAPTION= <scene name='initialview01'>1ted</scene>, resolution 2.25Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MYR:MYRISTIC ACID'>MYR</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] | ||
| + | |GENE= pks18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a type III polyketide synthase PKS18 from Mycobacterium tuberculosis''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TED is a [ | + | 1TED is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TED OCA]. |
==Reference== | ==Reference== | ||
| - | A novel tunnel in mycobacterial type III polyketide synthase reveals the structural basis for generating diverse metabolites., Sankaranarayanan R, Saxena P, Marathe UB, Gokhale RS, Shanmugam VM, Rukmini R, Nat Struct Mol Biol. 2004 Sep;11(9):894-900. Epub 2004 Aug 1. PMID:[http:// | + | A novel tunnel in mycobacterial type III polyketide synthase reveals the structural basis for generating diverse metabolites., Sankaranarayanan R, Saxena P, Marathe UB, Gokhale RS, Shanmugam VM, Rukmini R, Nat Struct Mol Biol. 2004 Sep;11(9):894-900. Epub 2004 Aug 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15286723 15286723] |
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Naringenin-chalcone synthase]] | [[Category: Naringenin-chalcone synthase]] | ||
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[[Category: thiolase fold]] | [[Category: thiolase fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:17:22 2008'' |
Revision as of 12:17, 20 March 2008
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| , resolution 2.25Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | pks18 (Mycobacterium tuberculosis) | ||||||
| Activity: | Naringenin-chalcone synthase, with EC number 2.3.1.74 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a type III polyketide synthase PKS18 from Mycobacterium tuberculosis
Overview
The superfamily of plant and bacterial type III polyketide synthases (PKSs) produces diverse metabolites with distinct biological functions. PKS18, a type III PKS from Mycobacterium tuberculosis, displays an unusual broad specificity for aliphatic long-chain acyl-coenzyme A (acyl-CoA) starter units (C(6)-C(20)) to produce tri- and tetraketide pyrones. The crystal structure of PKS18 reveals a 20 A substrate binding tunnel, hitherto unidentified in this superfamily of enzymes. This remarkable tunnel extends from the active site to the surface of the protein and is primarily generated by subtle changes of backbone dihedral angles in the core of the protein. Mutagenic studies combined with structure determination provide molecular insights into the structural elements that contribute to the chain length specificity of the enzyme. This first bacterial type III PKS structure underlines a fascinating example of the way in which subtle changes in protein architecture can generate metabolite diversity in nature.
About this Structure
1TED is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
A novel tunnel in mycobacterial type III polyketide synthase reveals the structural basis for generating diverse metabolites., Sankaranarayanan R, Saxena P, Marathe UB, Gokhale RS, Shanmugam VM, Rukmini R, Nat Struct Mol Biol. 2004 Sep;11(9):894-900. Epub 2004 Aug 1. PMID:15286723
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